From 2007.igem.org

Abstract　　Concept & Model　　Requirements　　Genetic_circuit　　Works　　About_our_team

　　0. Hybrid promoter　　1. Formulation　　2. Assay1　　3. Simulation　　4. Assay2　　5. Future works

Approach

We have alternated/combined Wet and Dry experiments to achieve our goal

0. Wet :　Hybrid promoter

The newly deviced promoter sensed the TWO INPUTS, AHL & reperssor, as shown in Fig., working as an AND gate.

1.Dry :　 Formulation

簡単な式からわかることを導きながら、段階を経て今回必要な計算式を導出した。最終的な式では、確率微分方程式を用いている。

2.Wet : 　Assay1 using "Externally added" materials

We decided the parameter of hill coefficients by the assay of AHL & IPTG |

3.Dry : 　Simulation

Based on stochastic simulation using parameters obtained from our wet experiments, we have confirmed that the whole system becomes unstable when there are only idlers left, and then, they become either of workers and idlers. Also we have determined other parameters necessary for the desired behavior of this system.

4.Wet : 　Assay2 using "Cell-produced" AHL

By the wet experiments using E. coli, we testified whether our current plasmids could satisfy the other required conditions (for the desired behavior of the system) estimated by the simulation.

5.Future works

Based on the results from the wet experiments in the step 4, the next dry approach - analysis and simulation - should be performed, which will in turn be testified and confirmed by wet experiments. Continueing these processes, if time permits, would further sophisticate our model.