From 2007.igem.org

Projects

Project Design: Wet Lab

Project Design: Printer and Software

Testing

Constructing our Project: The Wetlab

Protocols

Final Result of E.co Lisa

Once we had chosed our project the next step was to design a system that would do what we wanted. The steps outlined in this section are the MOST CURRENT used in our project. This section does not describe the primers, plates, and parts that were considered but not used. Or the techniques that were attempted but did not work

E. coLisa Project Design

The following circut was designed to implement our system (image and design schematic pending)

E. coLisa Project Preparation Work

Once a design for the project had been determined our team begin preparing all of the materials and reagents needed to complete our project. The idea was to have everything ready to go for when the biobrick parts arrive.

Preparing Plates. AMP resistant

Have everything ready to go for when parts arrive

Quick list of parts...

• R0084 - Light Sensor Promoter
• R0062 - AHL Promoter
• R0011 - Temperature Sensitive Promotor
• S03600 - AHL Intermediate
• I13544 - GFP RBS Terminator
• J23008 - RNA Key
• B0034 - RBS
• B0015 - Terminator
• J06501 - Temperature Sensitive Component
• I51001 - ccdb and AMP resistant (death gene used to do plasmid switches

Wells from registery

I) Rehydrate the wells

II) 2 micro litres of rehydrated part --> transformed into TOP10