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Works top　　0.Hybrid promoter　　1.Formulation　　2.Assay1　　3.Simulation　　4.Assay2　　5.Future works

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Deviation of Hill function

1.Hill function for the activator AHL

Without the repression by LacI( [LacI]=0 ), Ex.6.1 was described as

Therefore, in the steady state the Hill function for the activator AHL was given as

2.Hill function for the repressor LacI

When the concentration of AHL was assumed to be infinity, Ex.6.1 was described as

Therefore, in the steady state the Hill function for the repressor LacI was given as

However, the amount of LacI cannot be actually measured.So,we used the inducer IPTG to calculate the Hill function for LacI.
Assume that the reaction between [LacI] and [IPTG] is the equilibrium reaction,these concentration in equilibrium state were shown in Fig.3.

Therefore,the equilibrium constant k was obtained as