Tokyo/Experimental precision and accurecy

From 2007.igem.org

Revision as of 11:14, 25 October 2007 by Hiro (Talk | contribs)

Contents

AHL assay

Purpose1:

To see how precisely we determine the fluorescence from GFP by removing LB media.

Samples:

placQI + GFP in pTrc99A (pos. con.)
A4 ΔP+ GFP (neg. con.)

Procedure:


AHL assay Standard protocol
Make 200 ul of mixtures of pos. con. and neg. con.
The mixture ratios of pos. con. and neg. con. are
(100:0), (70:30), (50:50), (30:70), (10:90), (3:97), (1:99)
Wash

Result & conclusion:

"Back to Preliminary assays"

Retrieved from "http://2007.igem.org/wiki/index.php/Tokyo/Experimental_precision_and_accurecy"