Freiburg07/In vivo test I
From 2007.igem.org
Revision as of 13:10, 25 October 2007 by Luckytaschka (Talk | contribs)
In vivo test I
Ca2+ stock: 100mM
| Ca2+ [µg/ml] | 0 | 0.1 | 1.0 | 10 |
| IPTG [µg/ml] | 0.5 | 0.5 | 0.5 | 0.5 |
| Amp [µg/ml] | 50 | 50 | 50 | 50 |
With this concentration of Ca2+, Amp and IPTG were following plates pour.
3 plates IPTG
3 plates IPTG + AMP
3 plates IPTG + Amp + Ca2+ (0.1)
3 plates IPTG + Amp + Ca2+ (1.0)
3 plates IPTG + Amp + Ca2+ (10)
3 plates IPTG + Ca2+ (0.1)
3 plates IPTG + Ca2+ (1.0)
3 plates IPTG + Ca2+ (10)
500 cells/100µl were plated in each plate.
