USTC

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Today = new Date(); Jamboree = new Date("November 3, 2007"); msInADay = 1000 * 60 * 60 * 24; display = Math.floor((Jamboree.getTime() - Today.getTime())/msInADay); document.write(" " + display +" "); // </script></html>days left until the Jamboree! </font> -->

Our Project:
Extensible Logic Circuit in Bacteria

Artificial Bio-Logic Circuit is composed of "logic gates" and "wires" like Digital Electronic Circuits. Though we have been enjoying the advantages of ultra-large-scale electronic circuits in modern life, we still cannot implement a somewhat small-scale circuit in vivo with several levels of gates.

Our project is to provide a new method for building up a fully extensible bio-logic circuit in bacteria. A small fragment of DNA containing cis-acting elements, favored for its small scale and potential to implement complex logic computation in vivo, can be systematically built up and act as a gate. Meanwhile, artificial repressors with highly-specific DNA-recognition regions are able to transmit signals without mutual interference, just as enameled wires. In this way, a circuit can be constructed regardless of the number of logic gates and the layout of the wires.

A demonstration system has also been assembled to show the practicality of this method. Just like Digital Electronic Circuits in early days, it is simple and ugly. Nevertheless, how will it appear in future?

For detailed description, please click each hyperlink below.

From left to right
Back row: Zhao Yun, Liu ZQ, Ma XY
Front row: Ma Rui, Su XF, Ding Bo, Zhan Jian

Project

Introduction

Core Logic Components:

Logic-Gate Promoters

Wires without Interference:

Repressor Evolution on Plates

Repressor Evolution in Silico

Peripheral Devices:

Inputs and Outputs

Extensible System:

A Demonstration

Further More

Members

4 Graduates:
Zhan Jian
Ma Rui
Ding Bo
Ma Xiaoyu

3 Undergraduates:
Su Xiaofeng
Liu Ziqing
Zhao Yun

3 Faculty Advisors:
Prof. HY Liu
Prof. JR Wu
Prof. ZH Hou

Statistics

In more than 4 months, our

Parts designed
= 229 parts . . . [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=USTC Go to see them]
Parts submitted
= 123 parts

Oligo-synthesis
~ 120 fragments
PCR synthesizing and detection
~ 800 times
Restriction digestion
> 600 times
Transformation
~ 300 times

DNA sequencing
~ 130 times
Fluorescence detection
> 100 times
Fluorescent Density measuring
> 300 times
ONPG assay
~ 100 times

Events