Chiba/Communication
From 2007.igem.org
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Introduction | Project Design ( 1.Sticky Hands | 2.Communication | 3.Size Control ) | Making Marimos | Our Goal || Team Members | メンバ連絡簿 |
Communication Unit
Our Aim
Our aim is to make bacteria stick around a bacteria core.
Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal.
Our system works like this: Sender core, which is sticking each other in advance, sends a signal to receivers, which grow stickey tags.
Then they stick together. We use Quorum sensing as a signal.
About Quorum Sensing
Quorum sensing is a cell-to-cell signaling action of bacteria. They detect the cell density of the same species and coordinate the expression behavior of their cells. Species of Gram-Negative signaling transfer molecules (so-called autoinducer) is a series of acyl homoserine lactone (AHL). When the AHL concentration exceeds a threshold, that is, the cell population is over a certain number, the promoter plux transcription is activated.
Detailed gene circuit is discribed in Fig. 2.
- more about quorum sensing
[http://partsregistry.org/Featured_Parts:Cell-Cell-Signaling Cell-Cell-Signaling]
[http://www.che.caltech.edu/groups/fha/quorum.html About Quorum sensing]
Experiments: New Parts!!
Recievers
New Parts We Made!!
- AHL > GFP generator (constitutive aiiA) : [http://partsregistry.org/Part:BBa_I729006 BBa_T729006]
- AHL > GFP & aiiA generator : [http://partsregistry.org/Part:BBa_I729005 BBa_I729005]
- Sensitive AHL > GFP generator : [http://partsregistry.org/Part:BBa_I729004 BBa_I729004]
From Biobrick
- BBa_T9002
Future Work
- inverter-aiiA
Senders
New Parts We Made
- MetK Sender ( could not deposit to registry )
From Biobrick
- Sender(BBa_S03623)
Future Work
- Light Sensing Sender (Light -> AHL)
futureでLuxIもスイッチできた方が魅力的だと思うんだ。∵ReceiverにSenderを混ぜるのではなく，初めからReceiverとSenderを混ぜた状態で凝集させることができる。ex.)Photoswitch etc... by tashiro
