June 12

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Experiments

Microscopy

  • pT LuxI CFP (J22241) induced with aTc at 0,10,100 ng/mL and imaging done after 6,8,10 hrs.
  • K12Z1 induced with aTc at 0,10,100 ng/mL and imaging done after 6,8,10 hrs.

FACS

  • pL CFP (J22202) induced with IPTG for 0,1,10,50,100,1000 uM and fluorescence measured after 5 hrs.
  • K12Z1 fluorescence taken after 5 hours (Negative control).
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