From 2007.igem.org

Growth kinetics of w121 strain

We make an array to test growth of w121 on different growth media (LB, S0.2, S0.4, S0.6, S0.8), supplemented with different amount of DAP.

Kinetic Array :le titre ici
	1	2	3	4	5	6	7	8	9	10	11	12
A	H20	H20	H20	H20	H20	H20	H20	H20	H20	H20	H20	H20
B	H20	LB+0µM DAP	LB+13µM DAP	LB+17µM DAP	LB+20µM DAP	LB+23µM DAP	LB+27µM DAP	LB+30µM DAP	LB+33µM DAP	LB+37µM DAP	LB+40µM DAP	H20
C	H20	S0.2+0µM DAP	S0.2+13µM DAP	S0.2+17µM DAP	S0.2+20µM DAP	S0.2+23µM DAP	S0.2+27µM DAP	S0.2+30µM DAP	S0.2+33µM DAP	S0.2+37µM DAP	S0.2+40µM DAP	H20
D	H20	S0.4+0µM DAP	S0.4+13µM DAP	S0.4+17µM DAP	S0.4+20µM DAP	S0.4+23µM DAP	S0.4+27µM DAP	S0.4+30µM DAP	S0.4+33µM DAP	S0.4+37µM DAP	S0.4+40µM DAP	H20
E	H20	S0.4+0µM DAP	S0.4+13µM DAP	S0.4+17µM DAP	S0.4+20µM DAP	S0.4+23µM DAP	S0.4+27µM DAP	S0.4+30µM DAP	S0.4+33µM DAP	S0.4+37µM DAP	S0.4+40µM DAP	H20
F	H20	S0.6+0µM DAP	S0.6+13µM DAP	S0.6+17µM DAP	S0.6+20µM DAP	S0.6+23µM DAP	S0.6+27µM DAP	S0.6+30µM DAP	S0.6+33µM DAP	S0.6+37µM DAP	S0.6+40µM DAP	H20
G	H20	S0.8+0µM DAP	S0.8+13µM DAP	S0.8+17µM DAP	S0.8+20µM DAP	S0.8+23µM DAP	S0.8+27µM DAP	S0.8+30µM DAP	S0.8+33µM DAP	S0.8+37µM DAP	S0.8+40µM DAP	H20
H	H20	H20	H20	H20	H20	H20	H20	H20	H20	H20	H20	H20

The 96 plates is surrounded by H₂O in order to maintain humidity rate during acquisition of the kinetic. In each slot, 200µL of the growth medium (LB or S0.x) is mixed with 2µL of w121 culture grown ON, and with different amount of DAP (see table). The kinetic is measured during 20H, one point is acquired each 4min10s.