Paris/What's going on in the lab

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Revision as of 15:40, 13 July 2007 by Nicolas C. (Talk | contribs)

See the Notebook

Contents

What's new ?

We just received a first part of the oligos


To Do List

PCRs

In Progress

FstZ strain caracterisation

Transduction of the deletion of DapA from the strain w121 into MG1655 and MG1655 FtsZ TS84

The goal is, by transduction, to introduce DapA deletion into FtsZ TS strain

  • We managed to build a stock of efficient phages (stock of 12.7.7)
  • Transduction in progress

Transfection of 3 Biobricks

The goal is to extract DNA from the plates and make a stock of plasmid we need.

  • BBa_I0500: Inducible pBad/araC in pSB2K3 (KanR)
  • BBa_J23100: strong constitutive promoter in BBa_J61002 (AmpR)
  • BBa_B0015: double terminator (B0010-B0012) in pSB1AK3 (AmpR)