Paris/July 21
From 2007.igem.org
< Paris
Revision as of 17:26, 21 July 2007 by Eismoustique (Talk | contribs)
Digestion reactions
Digestion Products | |||||
---|---|---|---|---|---|
Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Description |
D9 | pJ23100 ES vector | pJ23100 (MP1.1 & MP1.2) | EcoI | SpeI | Vector with the J23100 promoter ready for insertion of a RBS/CDS backward-insert |
D10 | pJ23100 promoter fw-insert | pJ23100 (MP1.1 & MP1.2) | EcoI | SpeI | J23100 promoter ready for usage as a fw-insert |
D11 | pJ23107 ES vector | pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4) | EcoRI | SpeI | Vector with the J23107 promoter ready for insertion of a RBS/CDS backward-inser |
D12 | pJ23107 promoter fw-insert | pJ23100 (MP2.1, MP2.2, MP2.3 & MP2.4) | EcoRI | SpeI | J23107 promoter ready for usage as a fw-insert |
D13 | mRFP | pJ23100 MP2.2 | SpeI | PstI | mRFP RBS/CDS/T sequence, can it be used as a backward inser? |
D14 | Cre ORF | J61047 MP9.1 & MP9.2 | XbaI | PstI | Cre ORF extracted for usage as a bw-insert |
D14 | B0030 bw-vector | B0030 MP5.1 & MP5.2 | SpeI | PstI | bacward vector for cloning a CDS bw-insert after the B0030 RBS |
D15 | pSB1AK3 vector | B0015 MP3.1 & MP3.2 | EcoRI | PstI | pSB1AK3 open vector for BioBrick cloning |