Melbourne/primary Restriction enzymes
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Contents |
Physical data
- Description: NEB Restriction enzymes are used generally in the Lab.
| Enzyme | Cut site | 100% buffers | Cost | No of U | Part no/data | location | In store |
|---|---|---|---|---|---|---|---|
| SpeI | 5A^CTAGT3 | B2 | US$244 | 2500U @ 10,000U/ml | [http://www.neb.com/nebecomm/products/productR0140.asp NEB.R0133L] | ||
| HindIII | 5A^AGCTT3 | B2 | US$53 | 10,000 @ 20,000U/ml | [http://www.neb.com/nebecomm/products/productR0140.asp NEB.R0104S] | ||
| PstI | 5CTGCA^G3 | B3 | US$58 | 10,000 @ 20,000U/ml | [http://www.neb.com/nebecomm/products/productR0140.asp NEB.R0140S] | Lab Enz Freezer Box | YES |
| EcoRI | 5G^AATTC3 | B1,2,3&4 | AU$89 | 10,000 @ 20,000U/ml | [http://www.neb.com/nebecomm/products/productR0101.asp NEB.R0101S] | Lab Enz Freezer Box | YES |
| HaeII | 5RGCGC^Y3 | B4 & B2 | US$53 | 2,000U @ 20,000U/ml | [http://www.neb.com/nebecomm/products/productR0107.asp NEB.R0107S] | Igem Freezer box | YES |
| SalI | 5G^TCGAC3 | B3 | US$56 | 2,000U @ 20,000U/ml | [http://www.neb.com/nebecomm/products/productR0138.asp NEB.R0138S] | Lab Enz Freezer Box | YES |
| XbaI | 5T^CTAGA3 | B2 | US$63 | 3,000U @ 20,000U/ml | [http://www.neb.com/nebecomm/products/productR0145.asp NEB.R0145S] | Lab Enz Freezer Box | YES |
Y= (C or T), R= (A or G) [http://www.neb.com/nebecomm/tech_reference/single_code.asp Single letter code]
Double Digest Buffers
Lab Location
- Location see table
- Permision to use required? No
Store
- Available from Bio21 store? Yes
- location: [Media:melbourne Erefrigerator |E-refrigerator] Needs account card. (see Daisy).
Manufacturer:
- Manufacturer:NEB
- complete listing of available enzymes
- PhillipDodson 03:48, 1 July 2007 (EDT):Primary Reagent Template 1/July/2007
