Bologna University/Procedure

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< Bologna University
Revision as of 09:41, 3 August 2007 by Silvia.tamarri2 (Talk | contribs)
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1. [http://partsregistry.org/Help:IGEM_07_Parts_Kit Biobricks amplification]
2. Digestion reaction
3. Run on agarose 1% gel
4. DNA extraction from gel (QIAquick Gel Extraction kit – Ca No 28704)
5. Ligation reaction
6. Transformation
7. Miniprep (QIAprep Spin Miniprep kit - Ca No 27104 Qiagen)
8. Stock preparation in 40% glycerol and digestion for control
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