| Plasmids: MiniPrep Products
|
| Number
| Name
| Description
| Products in use
| Date
|
| MP1
| pJ23100 in J61002
| Strong constitutive promoter in J61002 (clones 1 + 2)
|
| July 15
|
| MP2
| pJ23107 in J61002
| Medium constitutive promoter in J61002 (clones 1 + 2)
| MP2.2
| July 15
|
| MP3
| B0015 in pSB1AK3
| Double terminator (clones 1 + 2)
| MP3.1
| July 16
|
| MP4
| I0500 in pSB2K3
| AraC pBAD (clones 1 + 2)
|
| July 16
|
| MP5
| BBa_B0030 in pSB1A2
| Strong RBS (clones 1 + 2)
|
| July 18
|
| MP6
| BBa_E0422 in pSB1A2
| ECFP (RBS+LVA+Term) (clones 1 + 2)
| MP6'.1, MP6'.2
| July 18, July 24
|
| MP7
| BBa_E0241 in pSB1A2
| PoPs to GFP converter (clones 1 + 2)
| MP7'.1
| July 18, July 24
|
| MP8
| BBa_E0840 in pSB1A2
| gfp tri-part; strong rbs (clones 1 + 2)
|
| July 18
|
| MP9
| BBa_J61047 in pSB1A2
| Cre ORF (clones 1 + 2)
| MP9'.1, MP9'.2
| July 18, July 24
|
| MP10
| BBa_I0500 in pSB2K3
| AraC pBAD (clones 1 + 2)
|
| July 20
|
| MP11
| BBa_pJ23107 in p61002
| Medium constitutive promoter in J61002 (clones 3 + 4)
| MP11'.3
| July 20, July 23
|
| MP12
| BBa_P1003 in pSB1A2
| KanR
|
| July 27
|
| L1
| BBa_I0500 in J61002
| construct with pBad promotor regulating expression of mRFP
| L1.2, L1.3, L1".1, L1".2
| July 25, July 27
|
| L2
| Lox66DapAE.coli in pSB1A2
| Insertion of lox66DapAE.coli construct as a biobrick in pSB1A2 plasmid
| L2.1, L2.2
| July 25
|
| L6
| BBa_B0030+BBa_J61047
| strong RBS(B0030)-Cre ORF
| L6.1, L6.2, L6.3, L6".1, L6".2
| July 25, July 27
|
| L12
| lox71-gfp in pSB1A2
| construction of the biobrick lox71-gfp
| L12.1 & L12.2
| July 30
|
| Digestion Products
|
| Number
| Product Name
| Matrix Name
| Enzyme 1
| Enzyme 2
| Size
| Description
| Product Left
| []ng/µL
| Date
|
| D1
| pJ23100
| pJ23100
| Spe1
| Pst1
| 2096
| Vector with the J23100 promoter ready for insertion of a RBS/CDS insert
|
|
| July 17
|
| D2
| mRFP
| pJ23100
| Spe1
| Pst1
| 887
|
|
|
| July 17
|
| D3
| pJ23107
| pJ23107
| Spe1
| Pst1
| 2096
| Vector with the J23107 promoter ready for insertion of a RBS/CDS insert
|
|
| July 17
|
| D4
| mRFP
| pJ23107
| Spe1
| Pst1
| 887
|
|
|
| July 17
|
| D5
| pJ23100
| pJ23100
| EcoR1
| Spe1
| 2925
| The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
|
|
| July 17
|
| D6
| pJ23107
| pJ23107
| EcoR1
| Spe1
| 2925
| The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
|
|
| July 17
|
| D7
| Lox66 DapA E.coli
| Lox66 DapA E.coli (PCR product)
| EcoR1
| Pst1
| 977
|
|
|
| July 17
|
| D8
| Lox66 DapA E.coli
| Lox66 DapA E.coli (PCR product)
| Xba1
| Pst1
| 962
|
|
|
| July 17
|
| D9
| pJ23100 ES vector
| pJ23100 (MP1.1 & MP1.2)
| EcoI
| SpeI
| 2925
| Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert)
|
|
| July 21
|
| D10
| pJ23100 promoter fw-insert
| pJ23100 (MP1.1 & MP1.2)
| EcoI
| SpeI
| 58
| J23100 promoter ready for use as a fw-insert
|
|
| July 21
|
| D11
| pJ23107 ES vector
| pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4)
| EcoRI
| SpeI
| 2925
| Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter
|
|
| July 21
|
| D12
| pJ23107 promoter fw-insert
| pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4)
| EcoRI
| SpeI
| 58
| J23107 promoter ready for usage as a fw-insert
|
|
| July 21
|
| D13
| mRFP
| pJ23107 (MP2.2, MP2.3 & MP2.4)
| SpeI
| PstI
| 887
| mRFP RBS/CDS/T sequence, can it be used as a backward insert? => yes but you will not be able to add any biobrick after it
|
|
| July 21
|
| D14
| Cre ORF
| J61047 MP9.1 & MP9.2
| XbaI
| PstI
| 1063
| Cre ORF extracted for usage as a bw-insert
| Ø D14.1
|
| July 21
|
| D15
| B0030 bw-vector
| B0030 MP5.1 & MP5.2
| SpeI
| PstI
| 2076
| bacward vector for cloning a CDS bw-insert after the B0030 RBS
|
|
| July 21
|
| D18
| BB dig. lox66DapAE.coli
| lox66-DapAE.coli P5 PCR product
| EcoRI
| PstI
| ~960
| PCR product digested for cloning as a BioBrick
| Ø
|
| July 21
|
| D19
| BB dig. lox66DapAsubtilis
| lox66-DapAsubtilis P6 PCR product
| EcoRI
| PstI
| ~960
| PCR product digested for cloning as a BioBrick
| Ø
|
| July 21
|
| D19'
| BB dig. lox66DapAsubtilis
| lox66-DapAsubtilis P6' PCR product
| EcoRI
| PstI
| ~960
| PCR product digested for cloning as a BioBrick
|
|
| July 30
|
| D20
| bw-insert lox66-DapAE.coli
| lox66-DapAE.coli P5 PCR product
| XbaI
| PstI
| ~960
| PCR product digested for usage as a backward insert
| Ø
|
| July 21
|
| D21
| bw-insert lox66-DapAsubtilis
| lox66-DapAsubtilis P6 PCR product
| XbaI
| PstI
| ~960
| PCR product digested for usage as a backward insert
| Ø
|
| July 21
|
| D21'
| bw-insert lox66-DapAsubtilis
| lox66-DapAsubtilis P6' PCR product
| XbaI
| PstI
| ~960
| PCR product digested for cloning as a BI
|
|
| July 30
|
| D22
| pSB1A2 open vector
| BBa_J61047 (MP9.1 & MP9.2)
| EcoI
| PstI
| Ø D22.1
| Open pSB1A2 vector for BioBrick EcoRI/PstI insertio
| D22.1 Ø
|
| July 23
|
| D23
| AraC/pBad promoter fw-insert
| BBa_I0500 (MP4.1 & MP4.2)
| EcoI
| SpeI
| 1233
| AraC/pBad promoter ready for use as a forward insert
| Ø D23.1
|
| July 23
|
| D24
| eCFP bw-insert
| BBa_E0422 (MP6.1 & MP6.2)
| XbaI
| PstI
|
| eCFP (RBS-OFR_LVA-T) ready for use as a backward insert
|
|
| July 23
|
| D25
| PoPs to GFP converter
| BBa_E0421 (MP7.1 & MP7.2)
| XbaI
| PstI
|
| PoPs to GFP converter ready for use as a backward insert
|
|
| July 23
|
| D26
| gfp-tripart
| BBa_E0840 (MP8.1 & MP8.2)
| XbaI
| PstI
|
| gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert
|
|
| July 23
|
| D27
| lox71-ftsZ bw-insert
| lox71-ftsZ PCR product P7
| XbaI
| PstI
| lox71-ftsZ(unmutated) ready for use as a backward insert
|
| Ø
|
| July 23
|
| D27'
| lox71-ftsZ bw-insert
| lox71-ftsZ PCR product P7
| XbaI
| PstI
|
| lox71-ftsZ(unmutated) ready for use as a backward insert
|
|
| July 30
|
| D28
| RBS-DapAColi BB
| P8 (RBS-DapAColi)
| EcoRI
| PstI
| ~950
| Biobrick
|
|
| July 25
|
| D29
| RBS-DapAColi
| P8 (RBS-DapAColi)
| XbaI
| PstI
| ~950
| Backward insert
|
|
| July 25
|
| D30
| RBS-DapASubtilis BB
| P9 (RBS-DapASubtilis)
| EcoRI
| PstI
| ~950
| Biobrick
|
|
| July 25
|
| D31
| RBS-DapASubtilis
| P9 (RBS-DapASubtilis)
| XbaI
| PstI
| ~950
| Backward insert
|
|
| July 25
|
| D32
| Lox71-FtsA-FtsZ
| P10 Lox71-FtsA-FtsZ
| XbaI
| PstI
| ~2600
| Backward insert - EcoRI site still presents : we can't use EcoRI
|
|
| July 25
|
| D33
| RBS(B0030)CreORF
| L6' (clone 1 & 2)
| XbaI
| PstI
|
| RBS-Cre digestion for use as a BI
|
|
| July 30
|
| D34
| lox66
| L9' lox66
| SpeI
| PstI
|
| Biobrick plasmid carrying lox66 digested as a BV
|
|
| July 30
|
| D35
| lox71
| L10' lox71
| SpeI
| PstI
|
| Biobrick plasmid carrying lox71 digested as a BV
|
|
| July 30
|
| D36
| lox71-KmR-lox66
| P11 lox71-KmR-lox66
| EcoRI
| PstI
|
| lox71-KmR-lox66 digested as a biobrick insert
|
|
| July 30
|
