BerkiGEM2007-AustinConstructionFile54

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Pre-biobricking modifications to I716091.
Quickchange AD0013F/R to get rid of EcoR1 site.
Quickchange AD0014/F/R to get rid of BglII site.
Quickchange AD0015F/R to get rid of XhoI site.

PCR AD0016F/R on modified I716091. Digest Bgl2/Xho1 and insert into 9145 plasmid.