NYMU Taipei/Lab Notes/2007 8 29
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- Plasmid extraction for [pOmpC+(RBS+EYFP)]
- pOmpC+(RBS+EYFP) 1-1, 1-2, 2, 3, 4, 5 (duplicate the first tube for even balance)
- Extract from 1.5ml LB medium to 20ul EB buffer
- Stored in -20°C
- EYFP spectral characteristics:
- Excitation: 514nm
- Emition: 527nm
- Liquid culture for MGC clones
- INS (human), IGFBP3 (human), IGFBP7 (human), IDE (mouse) for insulin project
- PUM1 (human), AKAP1 (human) for m.t. project
Who attend?
- 立翔
