Week 8
From 2007.igem.org
Revision as of 10:07, 3 September 2007 by Alice.pasini (Talk | contribs)
- 08/20/07
- [http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012] transformation.
- 08/21/07
- There aren't colonies for [http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012]. We can't amplify these biobricks.
- [http://partsregistry.org/Part:BBa_J04631 J04631] digestion.
- Band extraction.
- [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010])ligation.
- 08/22/07
- There are no colonies for [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010])
- 08/23/07
- A new [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation with a new protocol (3h 15°C and 1.2 ul T4 Ligase).
- [http://partsregistry.org/Part:BBa_R0051 R0051], [http://partsregistry.org/Part:BBa_I763012 I763012] digestion with Spe/Pst1.
- [http://partsregistry.org/Part:BBa_P0412 P0412], [http://partsregistry.org/Part:BBa_J04631 J04631] digestion with Xba/Pst1.
- Run and extraction from gel.
- Transformation of [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligations.
- 08/24/07
- We plan new amplifications.
