From 2007.igem.org

DapA Wanner deletion: pKD3/pKD4-DapA homologies PCR

• Material:
  

- pKD3/pKD4: 1 µL (30ng/60ng)
- Tampon: 10µL
- dNTP: 1µL (final 200µmol each)
- Taq: 0.5µL
- Water: 32.5µL
- Primer1: 33: 2.5µL (final 0.5µM)
- Primer2: 34: 2.5µL (final 0.5µM)

• PCR Program:
  

35 cycles:
- 95°C: 5min
- 95°C: 30s (X35)
- 55°C: 30s (X35)
- 72°C: 1min30 (X35)
- 72°C: 10 min
- 4°C: inf

• Quantity: ng/µL
  
• Expected size: bp

• After purification:
  

2µL of purification product (2µL of ladder)