From 2007.igem.org

The global demand and importance for cheap, available, and disease free blood substitutes is undisputed. There are currently no red blood cell substitutes approved for clinical use in the US or the UK, and whole blood is almost always in short supply. Underdeveloped countries that need blood the most simply don’t have the infrastructure to support donation and storage, in addition a sizeable fraction of the population are disease carriers. We are developing an innovative and cheap blood substitute based on engineered E. coli with all the critical properties of human erythrocytes. These include the ability to safely exist in the bloodstream, carry oxygen, and be stored for prolonged periods in a freeze-dried state.

==>too detailed, move to a later section:

section on chasis: to make it safer to inject into the human bloodstream, and by adding components for oxygen delivery. A modified lipopolysaccharide should significantly (1000-10000x) reduce sepsis activity in the human bloodstream. section on oxygen carrying: A hemoglobin mutated to increase OD50 to match that of natural human blood cells after diphosphoglycerase. Heme and cytochrome B5 and B5 reductase complement the hemoglobin. Additional chaperone proteins such as sodC, HPI-katG, map, and AHSP were added to increase hardiness and prolong the half-life of the E. coli in the bloodstream. We are also investigating myoglobin, and section on freeze-drying: potential freeze-drying to preserve E. coli, with OtsA, OtsB, thpA through D (trehalose - ots) (hydroxyectoine).

Support for Berkeley iGEM 2007 was generously provided by SynBERC and The Camille and Henry Dreyfus Foundation, Inc.

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Team Members Advisors John Dueber [http://www.openwetware.org/wiki/User:JCAnderson Christopher Anderson] [http://genomics.lbl.gov/ Adam Arkin] Jay Keasling Teaching Assistants Farnaz Nowroozi Amin Hajimorad Rickey Bonds Undergraduate Researchers Arthur Yu Austin Day David Tulga Kristin Doan Samantha Liang Vaibhavi Umesh Kristin Fuller High School Students Vincent Parker Nhu Nguyen Hannah Cole

Team Resources [http://spreadsheets.google.com/ccc?key=pUQEpr4ZqU9TxjeURaNm1Vw Oligo List Spreadsheet] [http://spreadsheets.google.com/ccc?key=pUQEpr4ZqU9TerIU8gSvKbQ CloneSaver Spreadsheet] [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Berkeley_UC Our BioBrick Parts] All construction files All sequencing files If you need an invitation to the spreadsheets, ask Sam. Tools and Guides [http://www.openwetware.org/wiki/Arking:JCAOligoTutorialHome Biobricks and Cloning Tutorials] [http://pir.georgetown.edu/pirwww/search/pairwise.shtml Pairwise Alignment Online] [http://align.genome.jp/ Multiple Sequence Alignment] [http://en.wikipedia.org/wiki/Help:Wikitext_examples Wiki Formatting Guide] Useful Links [http://openwetware.org/wiki/IGEM:UC_Berkeley/2006 UC Berkeley iGEM 2006 OpenWetWare] [http://parts2.mit.edu/wiki/index.php/University_of_California_Berkeley_2006 UC Berkeley iGEM 2006 wiki] iGEM wikis: [http://parts2.mit.edu/wiki/index.php/Main_Page 2006], 2007 [http://partsregistry.org/Main_Page Registry of Standard Biological Parts] Biobricks Parts Lists: [http://parts2.mit.edu/r/parts/partsdb/pgroup.cgi?pgroup=iGEM&group=iGEM_Berkeley 2005], [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2006&group=Berkeley 2006], [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Berkeley_UC 2007] [http://openwetware.org/wiki/Arking:JCAOligoTutorialHome Tutorials]

Team Notebooks John Dueber Notebook Christopher Anderson Notebook Farnaz Nowroozi Notebook Amin Hajimorad Notebook Rickey Bonds Notebook Keep your wiki notebooks, sequencing/construction logs, and the registry updated! Arthur Yu's 1337 Notebook Austin Day Notebook David Tulga's Notebook Kristin Doan Notebook Samantha's Notebook (June - July 19, 2007 Samantha's Notebook (July 20, 2007 - present) Vaibhavi Umesh Notebook Kristin Fuller Notebook Vincent Parker Notebook Nhu Nguyen Notebook Hannah Cole Notebook Setup