From 2007.igem.org

yesterday -- tomorrow

Contents 1 Preliminary work on w121 strain 2 Transduction of the DapA deletion into MG1655 failed... 3 Glycerol stock of Acinetobacter ADP1 4 Solid culture of Acinetobacter ADP1

Preliminary work on w121 strain

As previously observed, the w121 strain did not grow at all in the S0 medium... We supposed that their might be no nutriments left in the medium because we used a medium in which MG1655 grew ON... We will redo the experiment but this time, the medium will be recovered from exponential phase culture of MG1655

• We started a culture 150 ml of MG1655, and we take samples of the culture at different OD during exponential phase :
  • At OD = 0.2 : Centrifugation of 25ml of culture, filtration of the supernatant. ==> S0.2
  • At OD = 0.4 : Centrifugation of 25ml of culture, filtration of the supernatant. ==> S0.4
  • At OD = 0.6 : Centrifugation of 25ml of culture, filtration of the supernatant. ==> S0.6
  • At OD = 0.8 : Centrifugation of 25ml of culture, filtration of the supernatant. ==> S0.8

Transduction of the DapA deletion into MG1655 failed...

We will redo the stock of P1 phages.

Glycerol stock of Acinetobacter ADP1

See protocols

Solid culture of Acinetobacter ADP1

We spread Acinetobacter on agar LNMM with and without Nile Red.
We spread E.Coli DH5alpha transformed by pKS::DGAT or not on agar LNMM with and without Nile Red.

See July6 for the results.

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