The numbers start at where the Arkin Wiki left off.
| Sample | Date | Plasmid | Clone | Primer | Result | File Link
|
| ald123 | 6/6/07 | 9145-I716053 | N/A | ca998 | perfect | ald123
|
| ald124 | 6/6/07 | 9145-I716054 | N/A | ca998 | perfect | ald124
|
| ald125 | 6/6/07 | 9145-I716055 | #1 | ca998 | wrong | ald125
|
| ald126 | 6-7-07 | 9145-I716055 | #2 | ca998 | perfect | ald126
|
| ald127 | 6-7-07 | pBCA1101-bca9231 | | ca998 | | ald127
|
| ald128 | 6-9-07 | I716058 AKA:I716053m29 | #1 | ca998 | perfect, point muation in EcoR1 Site? | ald128
|
| ald129 | 6-9-07 | I716058 AKA:I716053m29 | #2 | ca998 | Deletion following mutation | ald129
|
| ald130 | 6-9-07 | I716058 AKA:I716053m29 | #3 | ca998 | perfect, point muation in EcoR1 Site? | ald130
|
| ald131 | 6-9-07 | I716059 AKA:I716054m108 | #1 | ca998 | Perfect | ald131
|
| ald132 | 6-9-07 | I716059 AKA:I716054m108 | #2 | ca998 | Perfect | ald132
|
| ald133 | 6-9-07 | I716059 AKA:I716054m108 | #3 | ca998 | Perfect | ald133
|
| ald134 | 6-14-07 | 9145-Bca1154 | #1 | ca998 | (Revised: Mixed plasmid) silent point mutation, Gln caa -> cag, %15.33 -> %28.92 | ald134
|
| ald135 | 6-14-07 | 9145-Bca1154 | #2 | ca998 | 2 point mutations, but were close calls. Probably correct | ald135
|
| ald136 | 6-14-07 | 9145-Bca1150 | #1 | ca998 | perfect | ald136
|
| ald137 | 6-14-07 | 9145-Bca1150 | #2 | ca998 | wrong | ald137
|
| ald138 | 6-14-07 | pTrc-I716061 | #1 | ca998 | wrong oligo... | ald138
|
| ald139 | 6-14-07 | pTrc-I716061 | #2 | ca998 | wrong oligo... | ald139
|
| ald140 | 6-14-07 | pTrc-I716061 | #3 | ca998 | wrong oligo... | ald140
|
| ald141 | 6-14-07 | pTrc-I716061 | #4 | ca998 | wrong oligo... | ald141
|
| ald142 | 6-14-07 | 9145-I716062 | #1 | ca998 | perfect | ald142
|
| ald143 | 6-14-07 | 9145-I716062 | #2 | ca998 | perfect | ald143
|
| ald144 | 6-14-07 | 9145-I716062 | #3 | ca998 | wrong | ald144
|
| ald145 | 6-14-07 | 9145-I716063 | #1 | ca998 | huge deletion | ald145
|
| ald146 | 6-14-07 | 9145-I716063 | #2 | ca998 | huge deletion | ald146
|
| ald147 | 6-14-07 | 9145-I716063 | #3 | ca998 | silent point mutation, T->A, Val 18.33% -> Val 10.88% | ald147
|
| ald148 | 6-18-07 | 9145-I716061 | #1 | D57 | Perfect (shows beta HbA) | ald148
|
| ald149 | 6-18-07 | 9145-I716061 | #2 | D57 | Perfect (shows alpha HbA) | ald149
|
| ald150 | 6-18-07 | 9145-I716061 | #1 | D58 | Perfect | ald150
|
| ald151 | 6-18-07 | 9145-I716061 | #2 | D58 | Perfect | ald151
|
| ald152 | 6-19-07 | 9145-1154 | #3 | ca998 | Wrong | ald152
|
| ald153 | 6-19-07 | 9145-1154 | #4 | ca998 | Failed | ald153
|
| ald154 | 6-22-07 | 9145-1154 | #1a | ca998 | point mutation K96R | ald154
|
| ald155 | 6-22-07 | 9145-1154 | #1b | ca998 | point mutation K96R | ald155
|
| ald156 | 6-22-07 | 9145-1154 | #1c | ca998 | point mutation K96R | ald156
|
| ald157 | 6-26-07 | 9145-I716070 | #1 | ca998 | Perfect! | ald157
|
| ald158 | 6-26-07 | 9145-I716070 | #2 | ca998 | Perfect! | ald158
|
| ald159 | 6-26-07 | 9145-I716070 | #3 | ca998 | Perfect! | ald159
|
| ald160 | 6-26-07 | pTrc-I716069 | #1 | D57 | Silent point mutation at nucleotide 103, beta chain, otherwise perfect | ald160
|
| ald161 | 6-26-07 | pTrc-I716069 | #1 | D58 | Silent point mutation at nucleotide 103, beta chain, otherwise perfect | ald161
|
| ald162 | 6-26-07 | pTrc-I716069 | #2 | D57 | Silent point mutation at nucleotide 103, beta chain, otherwise perfect | ald162
|
| ald163 | 6-26-07 | pTrc-I716069 | #2 | D58 | Silent point mutation at nucleotide 103, beta chain, otherwise perfect | ald163
|
| ald164 | 6-27-07 | 9145-I716073 | #1 | ca998 | Wrong | ald164
|
| ald165 | 6-27-07 | 9145-I716073 | #2 | ca998 | Wrong | ald165
|
| ald166 | 6-27-07 | 9145-I716073 | #3 | ca998 | Silent point mutation! | ald166
|
| ald167 | 6-27-07 | 9145-I716072 | #1 | ca998 | Wrong | ald167
|
| ald168 | 6-27-07 | 9145-I716072 | #2 | ca998 | Wrong | ald168
|
| ald169 | 6-27-07 | 9145-I716072 | #3 | ca998 | wrong | ald169
|
| ald170 | 6-27-07 | 9145-I716068 | #1 | ca998 | Point deletion | ald170
|
| ald171 | 6-27-07 | 9145-I716068 | #2 | ca998 | Wrong | ald171
|
| ald172 | 6-27-07 | 9145-I716068 | #3 | ca998 | Wrong | ald172
|
| ald173 | 7-01-07 | 9145-I716068 | 1a | ca998 | Perfect | ald173
|
| ald174 | 7-01-07 | 9145-I716068 | 2a | ca998 | Point deletion, but probably not, near the end of read and it's kind of fuzzy | ald174
|
| ald175 | 7-01-07 | 9145-I716068 | 5a | ca998 | Point mutation and is parent vector from the previous step. (No mutation) | ald175
|
| ald176 | 7-01-07 | 9145-I716068 | 6a | ca998 | Perfect | ald176
|
| ald177 | 7-01-07 | 9145-I716068 | 8a | ca998 | Perfect | ald177
|
| ald178 | 7-04-07 | 9145-I716072 | 1 | ca998 | Perfect | ald178
|
| ald179 | 7-04-07 | 9145-I716072 | 2 | ca998 | Perfect | ald179
|
| ald180 | 7-04-07 | 9145-I716072 | 3 | ca998 | Perfect | ald180
|
| ald181 | 7-04-07 | 9145-I716072 | 4 | ca998 | Perfect | ald181
|
| ald182 | 7-04-07 | 9145-I716072 | 5 | ca998 | Wrong | ald182
|
| ald183 | 7-04-07 | 9145-I716072 | 6 | ca998 | Perfect | ald183
|
| ald184 | 7-04-07 | 9145-I716077 | 1 | ca998 | The point mutation changed back, still silent (Didn't "really" change back) otherwise perfect | ald184
|
| ald185 | 7-04-07 | 9145-I716077 | 2 | ca998 | Perfect until the read goes bad (probably should start sequencing backwards too) | ald185
|
| ald186 | 7-04-07 | 9145-I716078 | 1 | ca998 | Perfect | ald186
|
| ald187 | 7-04-07 | 9145-I716078 | 2 | ca998 | Perfect | ald187
|
| ald188 | 7-04-07 | 9145-I716079 | 1 | ca998 | Perfect | ald188
|
| ald189 | 7-04-07 | 9145-I716079 | 2 | ca998 | Perfect until read goes bad. | ald189
|
| ald190 | 07-09-07 | 9145-I716011 | | ca998 | Perfect | ald190
|
| ald191 | 07-09-07 | 9145-I716011 | | g00101 | Perfect | ald191
|
| ald192 | 07-09-07 | 9145-I716071 | | ca998 | Wrong, missing alpha subunit | ald192
|
| ald193 | 07-09-07 | 9145-I716071 | | g00101 | Wrong, missing alpha subunit | ald193
|
| ald194 | 07-09-07 | 9145-I716074 | | ca998 | Wrong, missing alpha subunit | ald194
|
| ald195 | 07-09-07 | 9145-I716074 | | g00101 | Wrong, missing alpha subunit | ald195
|
| ald196 | 07-09-07 | 9145-I716075 | | ca998 | Wrong, missing alpha subunit | ald196
|
| ald197 | 07-09-07 | 9145-I716075 | | g00101 | Wrong, missing alpha subunit | ald197
|
| ald198 | 07-09-07 | 9145-I716076 | | ca998 | Wrong, missing alpha subunit | ald198
|
| ald199 | 07-09-07 | 9145-I716076 | | g00101 | Wrong, missing alpha subunit | ald199
|
| ald200 | 07-09-07 | 9145-I716083 | #2 | D57 | Wrong | ald200
|
| ald201 | 07-09-07 | 9145-I716084 | #2 | D57 | Wrong construct, has providence and the 2X alpha subunit | ald201
|
| ald202 | 07-09-07 | 9145-I716085 | #2 | D57 | Failed read: Multiple Plasmids | ald202
|
| ald203 | 07-09-07 | 9145-I716086 | #2 | D57 | Missing Alpha Subunit | ald203
|
| ald204 | 07-13-07 | 9145-I716080 | | ca998 | Perfect | ald204
|
| ald205 | 07-13-07 | 9145-I716081 | | ca998 | Perfect | ald205
|
| ald206 | 07-13-07 | 9145-I716082 | | ca998 | Silent point mutation, so ~Perfect! (Phew!) | ald206
|
| ald207 | 7-16-07 | 9145-I716074 | | ca998 | Beta part is good, but lots of background | ald207
|
| ald208 | 7-16-07 | 9145-I716074 | | g00101 | can see the end of an alpha, but can't tell if its a di-alpha. Terminator perfect | ald208
|
| ald209 | 7-16-07 | 9145-I716075 | | ca998 | Can see perfect beta | ald209
|
| ald210 | 7-16-07 | 9145-I716075 | | g00101 | Can see perfect terminator and the link between the alpha subunits, lots of "point mutations" due to a mixed template, will proceed with this | ald210
|
| ald211 | 7-16-07 | 9145-I716076 | | ca998 | Can see beta perfectly | ald211
|
| ald212 | 7-16-07 | 9145-I716076 | | g00101 | Term and most of alpha present. "point mutations" due to mixed read, but seems good enough to proceed | ald212
|
| ald213 | 7-17-07 | 9145-I716084 | | ca998 | Shows promoter | ald213
|
| ald214 | 7-17-07 | 9145-I716085 | | ca998 | Shows promoter | ald214
|
| ald215 | 7-17-07 | 9145-I716086 | | ca998 | Shows promoter | ald215
|
| ald216 | 7-17-07 | 9145-I716071 | | ca998 | Mixed template, but almost perfect | ald216
|
| ald217 | 7-17-07 | 9145-I716071 | | g00101 | Mixed template, but almost perfect | ald217
|
| ald218 | 7-18-07 | 9145-I716083 | | ca998 | Wrong, sucka. (I think I overloadded the lane, will do again) | ald218
|
| ald219 | 7-19-07 | 9145-I716084 | 1c | ca998 | Gene is gone?!! | ald219
|
| ald220 | 7-19-07 | 9145-I716084 | 1c | g00101 | Gene is gone?!! | ald220
|
| ald221 | 7-19-07 | 9145-I716085 | 1c | ca998 | Very badly mixed read | ald221
|
| ald222 | 7-19-07 | 9145-I716085 | 1c | g00101 | Very badly mixed read | ald222
|
| ald223 | 7-19-07 | 9145-I716086 | 1c | ca998 | Very badly mixed read | ald223
|
| ald224 | 7-19-07 | 9145-I716086 | 1c | g00101 | Very badly mixed read | ald224
|
| ald225 | 7-23-07 | 9145-I716083 | 3 | ca998 | Perfect | ald225
|
| ald226 | 7-23-07 | 9145-I716083 | 4 | ca998 | Perfect | ald226
|
| ald227 | 7-23-07 | 9145-I716084 | 1 | ca998 | Silent Point Mutation | ald227
|
| ald228 | 7-23-07 | 9145-I716084 | 3 | ca998 | Damn wrong | ald228
|
| ald229 | 7-23-07 | 9145-I716085 | 1 | ca998 | Point mutation! Damn it!! V->A | ald229
|
| ald230 | 7-23-07 | 9145-I716086 | 1 | ca998 | Perfecto! | ald230
|
| ald231 | 7-23-07 | 9145-I716086 | 3 | ca998 | Damn wrong | ald231
|
| ald232 | 7-23-07 | 9145-I716086 | 2z | ca998 | Perfecto! | ald232
|
| ald233 | 7-23-07 | 9145-I716083 | 3 | g00101 | Perfect | ald233
|
| ald234 | 7-23-07 | 9145-I716083 | 4 | g00101 | Perfect | ald234
|
| ald235 | 7-23-07 | 9145-I716084 | 1 | g00101 | Silent point mutation | ald235
|
| ald236 | 7-23-07 | 9145-I716084 | 3 | g00101 | Damn wrong | ald236
|
| ald237 | 7-23-07 | 9145-I716085 | 1 | g00101 | Point mutation! Damn it!! V->A | ald237
|
| ald238 | 7-23-07 | 9145-I716086 | 1 | g00101 | Perfecto! | ald238
|
| ald239 | 7-23-07 | 9145-I716086 | 3 | g00101 | Damn wrong | ald239
|
| ald240 | 7-23-07 | 9145-I716086 | 2z | g00101 | Perfecto! | ald240
|
| ald241 | 7-26-07 | 9145-I716085 #1 cpcr | | ca998 | Read sucks, can't see link between dialpha, but still may be right if quintarabio got their act together | ald241
|
| ald242 | 7-26-07 | 9145-I716085 #2 cpcr | | ca998 | Can see link between dialpha and whole beta, looks perfect | ald242
|
| ald243 | 7-26-07 | 9145-I716085 #1 cpcr | | g00101 | Read sucks, can't see link between dialpha, but still may be right if quintarabio got their act together | ald243
|
| ald244 | 7-26-07 | 9145-I716085 #2 cpcr | | g00101 | Can see link between dialpha and whole beta, looks perfect | ald244
|
| ald245 | 7-30-07 | petDUET-I716091 | 1 | AD0010F | Perfect | ald245
|
| ald246 | 7-30-07 | petDUET-I716091 | 1 | AD0011F | Perfect | ald246
|
| ald247 | 7-30-07 | petDUET-I716093 | 2-1 | AD0010F | Perfect beta and up to di-alpha linkage | ald247
|
| ald248 | 7-30-07 | petDUET-I716094 | 1-3 | AD0010F | Perfect | ald248
|
| ald249 | 7-30-07 | petDUET-I716094 | 1-3 | AD0011F | Perfect | ald249
|
| ald250 | 8-14-07 | petDUET-I716351 | 1 | g00101 | Shows little bit of cytochrome cassette | ald250
|
| ald251 | 8-14-07 | petDUET-I716351 | 1 | ca998 | Shows little bit of hem cassette | ald251
|
| ald252 | 8-14-07 | petDUET-I716353 | 2 | AD0012F | So very wrong (They said they used ca998 and g00101 though...) | ald252
|
| ald253 | 8-14-07 | petDUET-I716353 | 2 | AD0012R | So very wrong (They said they used ca998 and g00101 though...) | ald253
|
| ald254 | 8-15-07 | petDUET-I716092 | 3 | ca998 | Wrong oligos...my bad | ald254
|
| ald255 | 8-15-07 | petDUET-I716092 | 3 | g00101 | Wrong oligos...my bad | ald255
|
| ald256 | 8-22-07 | petDUET-I716353 | #1 | AD0016R | Can see the beginning of ADHE! But there is a 5 base pair insertion between the rbs and the ORF | ald256
|
| ald257 | 8-22-07 | petDUET-I716353 | #2 | AD0016R | Can see the beginning of ADHE! | ald257
|
| ald258 | 8-22-07 | 9145-I716354 2-1 | #2 | ca998 | Perfect beta | ald258
|
| ald259 | 8-22-07 | 9145-I716354 2-1 | #2 | g00101 | Perfect alpha | ald259
|
| ald260 | 8-22-07 | 9145-I716356 1-2 | | ca998 | Perfect beta and beginning of di alpha | ald260
|
| ald261 | 8-22-07 | 9145-I716356 1-2 | | g00101 | Can see dialpha linkage. Point mutation (that I called earlier) "changed back", silent mutation | ald261
|
| ald262 | 8-22-07 | 9145-I716357 1-1 | | ca998 | Bad read: Can only see beginning of beta. Looks good though. | ald262
|
| ald263 | 8-22-07 | 9145-I716357 1-1 | | g00101 | Perfect alpha, can see end of beta | ald263
|
| ald264 | 8-22-07 | 9145-I716357 2-1 | | ca998 | Questionable point mutation | ald264
|
| ald265 | 8-22-07 | 9145-I716357 2-1 | | g00101 | Questionable point mutation | ald265
|
| ald266 | 8-22-07 | petDUET-I716092 | 3 | AD0010F | Beta is there, really bad read though | ald266
|
| ald267 | 8-22-07 | petDUET-I716092 | 3 | AD0011R | di-alpha is there, very bad read. | ald267
|
| ald268 | 9-7-07 | I716366 | 1 | ca998 | Perfect | ald268
|
| ald269 | 9-7-07 | I716367 | 1 | ca998 | Perfect | ald269
|
| ald270 | 9-17-07 | I716362 | 1 | g00101 | sodc/katg are present | ald270
|
| ald271 | 9-17-07 | I716364 | 1 | g00101 | sodc/katg are present | ald271
|
| ald272 | 9-21-07 | I716368 | 2 | g00101 | Cytochromes present | ald272
|
| ald273 | 9-21-07 | I716369 | 2 | g00101 | Cytochromes present | ald273
|
| ald274 | 9-21-07 | T7-HemABCD-TT | 3 | g00101 | Terminator Present | ald274
|
| ald275 | 10-3-07 | I716276-277 | 1 | g00101 | Wrong, no AHSP | ald275
|
| ald276 | 10-3-07 | I716269-TT | 1 | g00101 | Can see TT-SodC-Ending of KatG | ald276
|
| ald277 | 10-3-07 | I716378 | 3 | AD0011F | Read goes bad too quick, but can see bits and pieces which match to the AHSP in correct position | ald277
|
| ald278 | 10-11-07 | I716379 | 2 | g00101 | Perfect AHSP addition | ald278
|
| ald279 | 10-11-07 | I716376 | 1 | g00101 | good | ald279
|
| ald280 | 10-11-07 | I716377 | 3 | g00101 | good | ald280
|
| ald281 | 10-11-07 | I716380+276+277 | 3 | g00101 | only metAP addition (only 277) | ald281
|
| ald282 | 10-11-07 | I716381+276+277 | 3 | g00101 | onlt metAP addition (only 277) | ald282
|
| ald283 | | | | | | ald283
|
| ald284 | | | | | | ald284
|
| ald285 | | | | | | ald285
|
| ald286 | | | | | | ald286
|
| ald287 | | | | | | ald287
|
| ald288 | | | | | | ald288
|
| ald289 | | | | | | ald289
|
| ald290 | | | | | | ald290
|
| ald291 | | | | | | ald291
|
| ald292 | | | | | | ald292
|
| ald293 | | | | | | ald293
|
| ald294 | | | | | | ald294
|
| ald295 | | | | | | ald295
|
| ald296 | | | | | | ald296
|
| ald297 | | | | | | ald297
|
| ald298 | | | | | | ald298
|
| ald299 | | | | | | ald299
|
| ald300 | | | | | | ald300
|
| ald301 | | | | | | ald301
|
| ald302 | | | | | | ald302
|
| ald303 | | | | | | ald303
|
| ald304 | | | | | | ald304
|
| ald305 | | | | | | ald305
|
| ald306 | | | | | | ald306
|
| ald307 | | | | | | ald307
|
| ald308 | | | | | | ald308
|
| ald309 | | | | | | ald309
|
| ald310 | | | | | | ald310
|
| ald311 | | | | | | ald311
|
| ald312 | | | | | | ald312
|
| ald313 | | | | | | ald313
|
| ald314 | | | | | | ald314
|
| ald315 | | | | | | ald315
|
| ald316 | | | | | | ald316
|
| ald317 | | | | | | ald317
|
| ald318 | | | | | | ald318
|
| ald319 | | | | | | ald319
|
| Sample | Date | Plasmid | Clone | Primer | Result | Link
|
| DT001 | 06/14/07 | 102 | 1 | F | Garbled Sequence | DT001
|
| DT002 | 06/14/07 | 102 | 2 | F | Garbled Sequence | DT002
|
| DT003 | 06/14/07 | 104 | 1 | F | Sample Mixup | DT003
|
| DT004 | 06/14/07 | 104 | 2 | F | Genomic DNA Framgent | DT004
|
| DT005 | 06/14/07 | 105 | 1 | F | Perfect! | DT005
|
| DT006 | 06/14/07 | 105 | 2 | F | RFP Parent Recombination A | DT006
|
| DT007 | 06/14/07 | 106 | 1 | F | Perfect! | DT007
|
| DT008 | 06/14/07 | 106 | 2 | F | RFP Parent Recombination A | DT008
|
| DT009 | 06/14/07 | 107 | 1 | F | RFP Parent Recombination A | DT009
|
| DT010 | 06/14/07 | 107 | 2 | F | Perfect! | DT010
|
| DT011 | 06/15/07 | 102 | 1 | F | Primer Dimer | DT011
|
| DT012 | 06/15/07 | 102 | 2 | F | Primer Dimer | DT012
|
| DT013 | 06/15/07 | 104 | 1 | F | Sample Mixup | DT013
|
| DT014 | 06/15/07 | 104 | 2 | F | Genomic DNA Framgent | DT014
|
| DT015 | 06/15/07 | 105 | 1 | F | Perfect! | DT015
|
| DT016 | 06/15/07 | 106 | 1 | F | Perfect! | DT016
|
| DT017 | 06/15/07 | 107 | 2 | F | Perfect! | DT017
|
| DT018 | 06/19/07 | 103 | 2 | F | Genomic DNA Framgent | DT018
|
| DT019 | 06/19/07 | 103 | 4 | F | Genomic DNA Framgent | DT019
|
| DT020 | 06/26/07 | 102 | 1 | F | Probably Correct (Read quality at end is low) | DT020
|
| DT021 | 06/26/07 | 102 | 2 | F | Right Gene - Too many mutations | DT021
|
| DT022 | 06/26/07 | 102 | 3 | F | Right Gene - Too many mutations | DT022
|
| DT023 | 06/26/07 | 102 | 4 | F | Probably Correct (Read quality at end is low) | DT023
|
| DT024 | 06/27/07 | 102 | 4 | R | Correct! (With mutations from template) | DT024
|
| DT025 | 07/02/07 | 103 | 2 | F | Probably Correct (Gene is much longer than read) | DT025
|
| | | | | | |
|
| DT026 | 07/02/07 | 103 | 4 | F | Probably Correct (Gene is much longer than read) | DT026
|
| DT027 | 07/02/07 | 104 | 2 | F | Probably Correct (Gene is much longer than read) | DT027
|
| DT028 | 07/02/07 | 104 | 3 | F | Probably Correct (Gene is much longer than read) | DT028
|
| DT029 | 07/03/07 | 103 | 2 | R | Probably Correct (Gene is much longer than read) | DT029
|
| DT030 | 07/03/07 | 103 | 4 | R | Probably Correct (Gene is much longer than read) | DT030
|
| DT031 | 07/03/07 | 104 | 2 | R | Probably Correct (Gene is much longer than read) | DT031
|
| DT032 | 07/03/07 | 104 | 3 | R | Probably Correct (Gene is much longer than read) | DT032
|
| DT033 | 07/10/07 | 105 | M-1 | F | Perfect! | DT033
|
| DT034 | 07/10/07 | 105 | M-2 | F | Perfect! | DT034
|
| DT035 | 07/13/07 | 103 | 3 | F | Probably Correct (Gene is much longer than read) | DT035
|
| DT036 | 07/13/07 | 103 | 4 | F | Correct, except for 1 mutation | DT036
|
| DT037 | 07/13/07 | 104 | 1 | F | Probably Correct (Gene is much longer than read) | DT037
|
| DT038 | 07/13/07 | 104 | 2 | F | Probably Correct (Gene is much longer than read) | DT038
|
| DT039 | 07/16/07 | 103 | 3 | R | Probably Correct (Gene is much longer than read) | DT039
|
| DT040 | 07/16/07 | 103 | 4 | R | Probably Correct (Gene is much longer than read) | DT040
|
| DT041 | 07/16/07 | 104 | 1 | R | Probably Correct (Gene is much longer than read) | DT041
|
| DT042 | 07/16/07 | 104 | 2 | R | Probably Correct (Gene is much longer than read) | DT042
|
| DT043 | 07/17/07 | 103 | 3 | dt011-T7-seq1 | Probably Correct (Gene is much longer than read) | DT043
|
| DT044 | 07/17/07 | 104 | 1 | dt011-T7-seq1 | Probably Correct (Gene is much longer than read) | DT044
|
| DT045 | 07/24/07 | 103 | 3 | dt012-T7-seq2 | Correct, except for 1 mutation | DT045
|
| DT046 | 07/24/07 | 104 | 1 | dt012-T7-seq2 | Correct! | DT046
|
| DT047 | 07/24/07 | 109 | 1 | ca1111F | Correct! | DT047
|
| DT048 | 07/24/07 | 109 | 2 | ca1111F | Correct! | DT048
|
| DT049 | 07/24/07 | 109 | 3 | ca1111F | Correct! | DT049
|
| DT050 | 07/24/07 | 109 | 4 | ca1111F | Correct! | DT050
|
| | | | | | |
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| DT051 | 07/30/07 | 115 | D4 | ca570F | Correct, has 1106H RBS (Used PCR) | DT051
|
| DT052 | 07/31/07 | 116(101) | 2 | F,dt003-pir-IR | Truncation Recombination (only ca998 worked) | DT052
|
| DT053 | 08/03/07 | 116 | 1 | F | Truncation Recombination | DT053
|
| DT054 | 08/03/07 | 116 | 2 | F | Truncation Recombination | DT054
|
| DT055 | 08/03/07 | 116 | 3 | F | Truncation Recombination | DT055
|
| DT056 | 08/03/07 | 116 | 4 | F | Truncation Recombination | DT056
|
| DT057 | 08/06/07 | 117 | 1 | ca570F | Correct! | DT057
|
| DT058 | 08/06/07 | 117 | 2 | ca570F | Correct! | DT058
|
| DT059 | 08/06/07 | 117 | 3 | ca570F | Correct! | DT059
|
| DT060 | 08/06/07 | 117 | 4 | ca570F | Correct! | DT060
|
| DT061 | 08/13/07 | 118 | 1 | R | Correct! | DT061
|
| DT062 | 08/13/07 | 118 | 2 | R | Appears Correct, but signal is too low | DT062
|
| DT063 | 08/18/07 | 119 | 1 | F | Correct! | DT063
|
| Sample | Date | Plasmid | Clone | Primer | Result | File Link
|
| KD001 | 6/12/07 | pBca9145-I716153 | hemB2 | ca998 | Ok, maybe short | KD001
|
| KD002 | 6/12/07 | pBca9145-I716153 | hemB4 | ca998 | Good, need to reverse sequence to get last 400bp | KD002
|
| KD003 | 6/12/07 | pBca9145-I716155 | hemD2 | ca998 | Too short | KD003
|
| KD004 | 6/12/07 | pBca9145-I716155 | hemD4 | ca998 | Good, need to reverse sequence to get last 200bp | KD004
|
| KD005 | 6/13/07 | pBca9145-I716153 | hemB4 | G101001 | Good, after reverse complementing, the first 100bp are off, but this part of the sequence is confirmed by KD002 | KD005
|
| KD006 | 6/13/07 | pBca9145-I716155 | hemD4 | G101001 | Good, after reverse complementing, the first 100bp are off, but this part of the sequence is confirmed by KD004 | KD006
|
| KD007 | 6/14/07 | pBca9145-I716151 | hemA3 | ca998 | Not good, lots of mutations | KD007
|
| KD008 | 6/14/07 | pBca9145-I716151 | hemA3 | G01001 | Not good, lots of mutations | KD008
|
| KD009 | 6/14/07 | pBca9145-I716154 | hemC1 | ca998 | Good | KD009
|
| KD010 | 6/14/07 | pBca9145-I716154 | hemC1 | G01001 | Good | KD010
|
| KD011 | 6/15/07 | pBca9145-I716151 | hemA2 | ca998 | GC-->CG, but in both hemA3 and hemA2 clones. Chris says we cloned a different template from one in pubmed and to proceed | KD011
|
| KD012 | 6/15/07 | pBca9145-I716151 | hemA2 | G01001 | Good | KD012
|
| KD013 | 6/15/07 | pBca9145-I716154 | hemC2 | ca998 | Good, need reverse sequence | KD013
|
| KD014 | 6/15/07 | pBca9145-I716154 | hemC2 | G01001 | Good | KD014
|
| KD015 | 6/20/07 | pBca9145-I716152 | hemAC1 | ca998 | Not good, some mutations | KD015
|
| KD016 | 20|07 | pBca9145-I716152 | hemAC1 | G01001 | Ok, but forward has mutations | KD016
|
| KD017 | 6/20/07 | pBca9145-I716152 | hemAC2 | ca998 | Good, need reverse sequence | KD017
|
| KD018 | 6/20/07 | pBca9145-I716152 | hemAC2 | ca998 | Good, need reverse sequence | KD018
|
| KD019 | 6/21/07 | pBca9145-I716152 | hemAC2 | G01001 | Good | KD019
|
| KD020 | 6/26/07 | pBca9145-I716161 | 1106A+hemA1 | ca998 | Good | KD020
|
| KD021 | 6/26/07 | pBca9145-I716162 | 1106A+hemAC2 | ca998 | Good | KD021
|
| KD022 | 6/26/07 | pBca9145-I716163 | 1106A+hemB2 | ca998 | Good | KD022
|
| KD023 | 6/26/07 | pBca9145-I716164 | 1106A+hemC2 | ca998 | No sequence, possibly problem with miniprep | KD023
|
| KD024 | 6/26/07 | pBca9145-I716165 | 1106A+hemD2 | ca998 | Good | KD024
|
| KD025 | 7/5/07 | pBca9145-I716167 | ShemAC+hemB1 | ca998 | Good | KD025
|
| Sample | Date | Plasmid | Clone | Primer | Result | File Link
|
| AY001 | 060407 | pBca9145-Bca1145 | #1 | ca998 | Probably good. Long, ambiguous long AAAAAAA. | AY001
|
| AY002 | 060407 | pBca9145-Bca1145 | #2 | ca998 | Good. | AY002
|
| AY003 | 061207 | pBca9145-HPI/katG (I716253) | A1 | ca998 | super wrong. Seems like only half of HPI/katG, from the AY004.Sal.katG.HPI-Fx "mutate GAT>GAC (BglII)" til the end, was cloned. | AY003
|
| AY004 | 061207 | pBca9145-HPI/katG (I716253) | A3 | ca998 | generally OK. There is one extra G at 584 in the goal plasmid in the calls file. There is low confidence, but I am not sure what to make of it. Possibly should send for recheck at this point; may be necessary to check the mutation point @1308, and the end portion as well. But based on the miniprep digest run yesterday, this guy has a high chance of being the right one. DECISION: sent as AY007 with G01001 for a reverse check. | AY004
|
| AY005 | 061207 | pBca9145-HPI/katG (I716253) | A4 | ca998 | crazy :( there are big gaps | AY005
|
| AY006 | 061207 | pBca9145-wbbL (I716000) | B1 | ca998 | also crazy | AY006
|
| AY007 | 061307 | pBca9145-HPI/katG (I716???) | A3 | G01001 | Looks good actually, only notable thing is silent point mutation @ 1782 TCG > TCA still S. but the mutation point was just missed by sequencing. I don't think it's necessary to recheck; i have faith the primers worked right. well.. idk. major concern still is the possible addition mutation of a G | AY007
|
| AY008 | 061307 | pBca9145-neuS (I716001) | C1 | ca998 | Forward. Looks great! | AY008
|
| AY009 | 061307 | pBca9145-neuS (I716001) | C1 | G01001 | Reverse. Looks great! | AY009
|
| AY010 | 061407 | I716002 (pBca9145-yfbE_promote) | D1 | ca998 | Perfect promoter cloned region. @ 443 there is a deletion of ttgcag. Point mutation @631, T > C | AY010
|
| AY011 | 061407 | I716002 (pBca9145-yfbE_promote) | D1 | G01001 | Mixed DNA tube (contamination) | AY011
|
| AY012 | 061407 | I716002 (pBca9145-yfbE_promote) | D4 | ca998 | Perfect promoter cloned region. @ 443 there is a deletion of ttgcag. Point mutation @631, T > C. @640 addition of a C | AY012
|
| AY013 | 061407 | I716002 (pBca9145-yfbE_promote) | D4 | G01001 | Mixed DNA tube (contamination) | AY013
|
| AY014 | 061507 | I716000 (pBca9145-wbbL) | E1 | ca998 | long stretch of t's at 696, sequencing got an extra one. Likely read error. Missing a T @ 812, but read quality is low here. Lack of agttgca right after insert?? maybe not?? | AY014
|
| AY015 | 061507 | I716000 (pBca9145-wbbL) | E2 | ca998 | long stretch of t's at 696, sequencing got an extra one. Likely read error. Missing a T at 801. Lack of agttgca right after insert?? maybe not?? | AY015
|
| AY016 | 061807 | I716000 (pBca9145-wbbL) | E1 | G01001 | good | AY016
|
| AY017 | 061807 | I716000 (pBca9145-wbbL) | E2 | G01001 | good | AY017
|
| AY018 | 061807 | I716253 (pBca9145-HPI/katG) | A3 | ay007 | good | AY018
|
| AY019 | 062107 | I716006 (pBca9145-Bca9203 B5red) | G1 | ca998 | fail, mixed sample?? I believe it's quintara's fault | AY019
|
| AY020 | 062107 | I716006 (pBca9145-Bca9203 B5red) | G3 | ca998 | fail, mixed sample?? I believe it's quintara's fault | AY020
|
| AY021 | 062507 | I716006 (pBca9145-Bca9203 B5red) | G1 | ca998 | looks good | AY021
|
| AY022 | 062507 | I716006 (pBca9145-Bca9203 B5red) | H3 | ca998 | looks good | AY022
|
| AY023 | 062507 | I716005 (pBca9145-Bca9229 cytoB5) | H3 | ca998 | looks good | AY023
|
| AY024 | 062507 | I716005 (pBca9145-Bca9229 cytoB5) | H4 | ca998 | looks good | AY024
|
| AY025 | 062807 | I716002 (pBca9145-yfbE_promote) | IB | ca998 | our proprietary yfbE was cloned successfully | AY025
|
| AY026 | 062807 | I716002 (pBca9145-yfbE_promote) | ID | ca998 | our proprietary yfbE was cloned successfully | AY026
|
| AY027 | 070507 | I716008 (Ptet-rbs-wbbL-rbs-neuS) | J1 | ca998 | wbbL or neuS not cloned. only Ptet. | AY027
|
| AY028 | 070507 | I716008 (Ptet-rbs-wbbL-rbs-neuS) | J2 | ca998 | wbbL or neuS not cloned. only Ptet. | AY028
|
| AY029 | 070507 | I716008 (Ptet-rbs-wbbL-rbs-neuS) | J3 | ca998 | Ptet and wbbL were successfully cloned. neuS, unsure. Possibly neuS is gone since there could be a BamHI site right after wbbL in sequence file. | AY029
|
| AY030 | 071107 | I716012 (101-008) | 1 | ca998 | Could not amplify | AY030
|
| AY031 | 071107 | I716012 (101-008) | 2 | ca998 | Could not amplify | AY031
|
| AY032 | 071207 | I716013 (pBca9145-yfbE-rbs-ATG) | 1 | ca998 | V>A right after BglII site. Amino acid change is bad. | AY032
|
| AY033 | 071207 | I716013 (pBca9145-yfbE-rbs-ATG) | 3 | ca998 | Perfection | AY033
|
| AY034 | 071207 | I716014 (pBca9145-yfbE_solo) | 1 | ca998 | Perfection | AY034
|
| AY035 | 071207 | I716014 (pBca9145-yfbE_solo) | 2 | ca998 | Perfection | AY035
|
| AY036 | 071207 | I716014 (pBca9145-yfbE_solo) | 3 | ca998 | Perfection | AY036
|
| AY037 | 071307 | I716015 (RFP no ATG) | 1 | ca998 | Perfection | AY037
|
| AY038 | 071307 | I716015 (RFP no ATG) | 2 | ca998 | Perfection | AY038
|
| AY039 | 071307 | I716015 (RFP no ATG) | 3 | ca998 | Perfection | AY039
|
| AY040 | 071307 | I716008 Ptet-wbbL-neuS | lib | ca998 | really weird I think it's missing half of Ptet | AY040
|
| AY041 | 071707 | I716008 Ptet-wbbL-neuS | lib | ca998 | really weird I think it's missing half of Ptet AGAIN - but the sequencing is extremely mixed. | AY041
|
| AY042 | 071907 | I716019 (t7-rbs-cytB5-rbs-cytB5red-dblterm) | lib | ca998 | T7 promoter, CytB5 and the first part of CytB5Red cloned successfully. 591 it is ambiguous if the base is right or not (CytB5Red) but data from Austin's I716011 sequence suggests that it is good. | AY042
|
| AY043 | 071907 | I716019 (t7-rbs-cytB5-rbs-cytB5red-dblterm) | lib | G01001 | dblTerm and the rest of CytB5Red was cloned successfully. | AY043
|
| AY044 | 072307 | I716008 | R1 | ca998 | half of ptet missing | AY044
|
| AY045 | 072307 | I716008 | R2 | ca998 | half of ptet missing | AY045
|
| AY046 | 072307 | I716008 | R3 | ca998 | half of ptet missing | AY046
|
| AY047 | 072307 | I716008 | R4 | ca998 | Ptet intact. RBS appears garbled but it's because N's were not placed in the I716008 for the library. Need to map to check for neuS. | AY047
|
| AY048 | 072307 | I716008 | R5 | ca998 | wtf is this lol | AY048
|
| AY049 | 072307 | I716008 | R6 | ca998 | half of ptet is missing | AY049
|
| AY050 | 072307 | I716008 | R7 | ca998 | wtf is this | AY050
|
