From 2007.igem.org

yesterday -- tomorrow

FtsZ^TS strain screening

We want to test these strains :

• 121.4
• 121.5
• 121.6
• 129
• 1.129
• DCR 14.1
• DCR 14.2
• DCR 14.3

I made glycerol stock of these strains, stored in the freezer. Test :

• Spread these strains on two plates (preheated before spreading) :
  • One at 30°C
  • One at 42°C

Results : every strain grew at 30 and 42°C => FAIL

Overnight Bacterial cultures

We launched several cultures :

• MG1655 -> to do transductions tomorrow (don't forget the DAP, David!)
• pJ23107_J61002 clones 3 & 4 in LB-Amp liquid; also striping clones 3-18 on LB-Amp Agar plates
• I0500_pSB2K3 clones 1 & 2 in LB-Kan liquid medium
• B0015_pSB1AK3 clones 3 & 4 in LB-Kan liquid medium

Tomorrow (20/07/07): perform transduction (MG1655), minipreps, glycerol stocks