From 2007.igem.org

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Contents 1 Week 9 2 Week 10 3 Week 11 4 Week 12

Week 9

19 Aug 2007

• Picked 3-4 transformants from "ligation" plates (17/8) and Grew up liquid cultures.
• Transformed the two ligation reactions in the pink rack in the cold room and plated on Gen + Amp plate.

20 Aug 2007

• Miniprepped liquid cultures from 19/8 labeled LA, LB, LC and P2 13K A, P2 13K B.
• Concentration of DNA was measured to be:
  A- 221ng/ul
  B-224ng/ul.
  

• Digested:
  1. LA X/S
  2. LB X/S
  3. LC X/S
  4. P2 13K A X/S
  5. P2 13K B X/S

Desired products:

• 1. 450bp (95bp different from CI-2 used as control)
  2. 450bp
  3. 450bp
  4. 1kb
  5. 1kb

• These were run on a gel.

21 Aug 2007

• Digestions were set up for ligations.

OmpR-inverter
-Vector: OmpR (AmpR) miniprepped P1 15P from 10/7 digested with S/P.
-Insert: c1 inverter (1kb fragment) P2 13K A with X/P.

RBS-LacZ
-Vector: LacZ (KanR) P2 9E from 6/8 with E/X.
-Insert: RBS (GenR) P4 8J from 6/7 with E/S.

Digested for 2h and heat inactivated for 10min at 80degC.

• Made Glycerol Stocks of P2 13K using dry ice method.

22 Aug 2007

• Picked 4 colonies from fresh ligation/transformation to Growing up.

- These transformations were made as follows:

• • Ligated
    1. 5ul of Purified insert from 17/8 (CI-2 E/S)
    2. 5ul of Redigested P1 5G with E/X and heat inactivated at 60degC for 10min.

• Recieved M30109 and CP919.

23 Aug 2007

24 Aug 2007

25 Aug 2007

Week 10

26 Aug 2007

27 Aug 2007

28 Aug 2007

29 Aug 2007

30 Aug 2007

31 Aug 2007

1 Sept 2007

Week 11

2 Sept 2007

3 Sept 2007

4 Sept 2007

5 Sept 2007

6 Sept 2007

7 Sept 2007

8 Sept 2007

Week 12

9 Sept 2007

10 Sept 2007

11 Sept 2007

12 Sept 2007

13 Sept 2007

14 Sept 2007

15 Sept 2007