Melbourne/Making glycerol Stocksl
From 2007.igem.org
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- Applications: Create a stock of recombinant bacteria that can be stored at -70C
- Time to complete protocol: 5min
- Lab time: 5 minutes
- Waiting time: 0
- Approximate cost of materials: $
Contents |
Method from primary and secondary reagents
Primary & secondary Reagents Required including controls
- Overnight bacterial culture
- Glycerol
- Liquid Nitrogen or Dry Ice and Ethanol
Method including controls
- Add to a sterile Screw Top tube
-800ul of overnight bacterial culture
-600ul of glycerol - Mix by inversion
- Immerse in liquid nitrogen and store in -70C freezer. Alternatively, cover the tube with dry ice and pour ethanol in the dry ice. Store immediately in -70C freezer.
Equipement Required
- Screw Top tube
- Pipettes