From 2007.igem.org

My Construction Files
My Sequencing Files
My Biobricks

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--Samanthaliang 15:29, 4 June 2007 (EDT)

• Oligos to make biobricks of toxins are not yet in - maybe Wednesday or Thursday?

For cassette between Cre and toxin: [rbs][ATG][Lox][Term][Lox]

• Made [rbs][ATG] variants with different ribosome binding sites

I716201 is Bca1106A.Bca1112

       I716202 is Bca1106B.Bca1112 

       I716203 is Bca1128A.Bca1112 

       I716204 is Bca1090.Bca1112 

• Made [Lox][Term] variants with 2 different terminators (will probably use Bca1092 version)

I716205 is Bca1114.Bca1124

      I716206 is Bca1114.Bca1092 

• Tomorrow will make [Lox][Term][Lox] part
• Then will put the rbsATG together with LoxTermLox

eek so many construction files and ape files to make.

to do