From 2007.igem.org
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Biobrickificreation of pir I716102 (R6K Origin Inducer)
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PCR pir-F/pir-IR on pir116 (375 bp, gp = A)
PCR pir-IF/pir-R on pir116 (599 bp, gp = B)
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PCR pir-F/pir-R on A+B (949 bp, BglII/XhoI)
Sub into pBca9145-Bca1144 (BglII/XhoI, 2063+910, L)
Product is pBca9145-I716102-pir
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dt001-pir-F Forward Cloning of pir ccaaaAGATCTatgagactcaaggtcatgatg
dt002-pir-IF Forward Removing the BglII site in pir gaagagttagaCcttaacattattg
dt003-pir-IR Reverse Removing the BglII site in pir caataatgttaagGtctaactcttc
dt004-pir-R Reverse Cloning of pir gctagCTCGAGttaGGATCCtcaccccttagcttttttgg
