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Construction of hemA basic part from E.Coli CFT073
PCR kd013/kd016 on hemA (547bp, gp=A)
PCR kd015/kd014 on hemA (761bp, gp=B)
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PCR kd013/kd014 on A+B (1288bp, Bg1II/XhoI)
Digest pBca9145-Bca1144#5 (BglII/XhoI, 2063+910, L)
Product is pBca9145-I716152
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kd013 Forward Bg1II for hemA ccaaaAGATCTatgacccttttagcactcggtatc
kd014 Reverse Xho1 for hemA gctagCTCGAGttaGGATCCctactccagcccgaggctgtc
kd015 Forward remove the Bg1II site in hemA gcgcggcAGATTTttgaatc
kd016 Reverse remove the Bg1II site in hemA gattcaaAAATCTgccgcgc
