Paris/October 16
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< Paris
Revision as of 15:25, 17 October 2007 by Eismoustique (Talk | contribs)
PCR rxn
the following PCR reactions have been performed
| PCR : PrefixBB-frt1-CmR-frt2-SufixBB | ||||||
|---|---|---|---|---|---|---|
| PCR Settings | Buffer (5x) | 5x 10µL (GC rich) | Expected size | |||
| Annealing (°C) | MgCl2 10µM | 10µM 0µL (no extra) | 1Kb | |||
| 0min | dNTP 10µM | 10µM 1µL | Success | |||
| Time Elongation | Oligo F 10µM | O51 | 10µM 4µL | YES | ||
| 1m40' | Oligo R 10µM | O52 | 10µM 4µL | Image (click to enlarge) | ||
| Number cycles | Water | qsp 50µL | [[Image:|30px]] | |||
| 30 | Polymerase | Phusion 0,5µL | Band (0=ladder) | |||
| DNA | pY-2P-IntC 5µL | |||||
| PCR : PrefixBB-frt1-CmR-frt2-T1T2-SufixBB | ||||||
|---|---|---|---|---|---|---|
| PCR Settings | Buffer (5x) | 5x 10µL (GC rich) | Expected size | |||
| Annealing (°C) | MgCl2 10µM | 10µM 0µL (no extra) | ||||
| 0min | dNTP 10µM | 10µM 1µL | Success | |||
| Time Elongation | Oligo F 10µM | O51 | 10µM 4µL | NO | ||
| 1m40' | Oligo R 10µM | O53 | 10µM 4µL | Image (click to enlarge) | ||
| Number cycles | Water | qsp 50µL | [[Image:|30px]] | |||
| 30 | Polymerase | Phusion 0,5µL | Band (0=ladder) | |||
| DNA | pY-2P-IntC 1µL (dil. 1/10) | |||||
| PCR : PrefixBB-mutDGAT-SufixBB | ||||||
|---|---|---|---|---|---|---|
| PCR Settings | Buffer (5x) | 5x 10µL (GC rich) | Expected size | |||
| Annealing (°C) | MgCl2 10µM | 10µM 0µL (no extra) | ||||
| 0min | dNTP 10µM | 10µM 1µL | Success | |||
| Time Elongation | Oligo F 10µM | O29 | 10µM 4µL | NO | ||
| 2m | Oligo R 10µM | O30 | 10µM 4µL | Image (click to enlarge) | ||
| Number cycles | Water | qsp 50µL | [[Image:|30px]] | |||
| 33 | Polymerase | Phusion 0,5µL | Band (0=ladder) | |||
| DNA | P13 1µL (dil. 1/10) | |||||
