Berkeley LBL/Digestion2

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< Berkeley LBL
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Digestion for Analysis (30 uL scale): 

      20 uL DNA

      3.0 uL appropriate NEB (10x)

      0.3 uL BSA (100x)

      1.0 uL Enzyme 1

      1.0 uL Enzyme 2

      Add H2O to total volume 30 uL

      30 uL total


  • Incubate at 37ºC for 2 hours
  • Add 0.5 uL of each enzyme
  • Incubate at 37 ºC for an additional 30 min.
  • Run analytic gel
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