BerkiGEM2007-SamanthaConstructionFile9

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Revision as of 00:09, 8 June 2007 by Samanthaliang (Talk | contribs)
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NOT DONE YET...found an ALWNI site in Cre, use BglI instead

[rbs][ATG]
4 Different rbs variants (1106A, 1106B, 1128A, 1090)
2 Different Cre variants (212 and 213)


Digest pBca1101-Bca1106A (BamHI/BglI, 1588+850+544bp, Large)
Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small)
Product is pBca9145-I716214.


Digest pBca1101-Bca1106B (BamHI/BglI, 1588+850+544bp, Large)
Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small)
Product is pBca9145-I716215.


Digest pBca1101-Bca1128A (BamHI/BglI, 1606+850+544bp, Large)
Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small)
Product is pBca9145-I716216.


Digest pBca9145-Bca1090 (BamHI/BglI, 1538+553+9bp, Large)
Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small)
Product is pBca9145-I716217.


Digest pBca1101-Bca1106A (BamHI/BglI, 1588+850+544bp, Large)
Digest pBca9145-I716213 (BglII/BglI, 2187+923bp, Small)
Product is pBca9145-I716218.


Digest pBca1101-Bca1106B (BamHI/BglI, 1588+850+544bp, Large)
Digest pBca9145-I716213 (BglII/BglI, 2187+923bp, Small))
Product is pBca9145-I716219.


Digest pBca1101-Bca1128A (BamHI/BglI, 1606+850+544bp, Large)
Digest pBca9145-I716213 (BglII/BglI, 2187+923bp, Small)
Product is pBca9145-I716220.


Digest pBca9145-Bca1090 (BamHI/BglI, 1538+553+9bp, Large)
Digest pBca9145-I716213 (BglII/BglI, 2187+923bp, Small)
Product is pBca9145-I716221.

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