BerkiGEM2007-SamanthaConstructionFile9
From 2007.igem.org
Revision as of 00:20, 8 June 2007 by Samanthaliang (Talk | contribs)
NOT DONE YET...found an ALWNI site in Cre, use BglI instead
[rbs][Cre]
rbs variants Bca1090
2 Different Cre variants (212 and 213)
Digest pBca9145-Bca1090 (BamHI/BglI/AlwNI, 942+596+553bp, Large)
Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small)
Product is pBca9145-I716217.
Digest pBca9145-Bca1090 (BamHI/BglI, 942+596+553bp, Large)
Digest pBca9145-I716213 (BglII/BglI, 2187+923bp, Small)
Product is pBca9145-I716221.
