NYMU Taipei/Protocols2
From 2007.igem.org
Wet-Lab Protocols
- [http://www.protocol-online.org/ Protocol online]
- PCR (7/20)
- RT-PCR
- Q-PCR
- Quantitation of Nucleic acid concentration
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- DNA Analysis through Gel Electrophoresis</li>
- Ligate blunt or cohesive ends in 5 minutes at room temperature
- Quick Ligation Kit (NEB)
- Fast Ligation Kit (UBI)
- Fast-Link DNA Ligation Kit (EPICENTRE Biotechnologies)
- Rapid Ligation Kit (Fermentas)
- All of these are just normal ligase buffers with PEG8000 at a 10-12% concentration. The NEB catalogue gives the original reference.
Use of crowding agents like PEG allow 5-minute sticky end ligations.
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- Vector construction
- Vector sequences
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- Transformation </li>
- Production of heterologous proteins in Escherichia coli </li>
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- Glucose assay
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- Mammalian Gene Collection (MGC) Full-length cDNA clones
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Dry-Lab Protocols
- serial cloner
- Protein Fusion Design
- rcsC-envZ cimaera design
- Twin-Arginine Translocation Pathway Signal Peptide
- Lee et al., 2006. The Bacterial Twin-Arginine Translocation Pathway. Annu. Rev. Microbiol., 60, p373-395
General references
