Kristin Doan Notebook
From 2007.igem.org
My Construction Files
My Sequencing Files
6/6/07
Today was a pretty easy day. We began with trying to continue the project that was very much unsuccessful yesterday. We wanted to make a composite part with the RFP insert downstream of the p-Tet promoter. We digested pBca9145-1144 to retrieve our RFP insert as well as pBca9145-9205, which contains the promoter. :( low and behold, we failed again. all the lanes with the RFP showed up beautifully, but no lanes showed up for the 9205 plasmid. BUT!! it may not be our fault because the 9205 DNA was never tested. who knows? so instead, we worked on our wikis (obviously) The day wasn't in total vain because I did read two articles on heme so I understand better now. yippee! Kdoan 17:37, 6 June 2007 (EDT)
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to do ==6/7/07