Week 16
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- 10/15/07
- O/N ligations for:
- -I763019;
- -pTet-LacI-T-Plambda-RFP.
- Run on electrophoresis gel and I763019 band extraction.
- 10/16/07
- Transformation of I763019 and pTet-LacI-T-Plambda-RFP.
- We inoculate:
- -I763005;
- -I763015;
- -I763004.
- 10/17/07
- Miniprep of:
- -I763005;
- -I763015;
- -I763004.
- We inoculate:
- -Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp;
- -pTet-LacI-T-pLambda-rfp (high copy).
éDigestion of
- 10/18/07
- 10/19/07