Freiburg07/primers

From 2007.igem.org

Revision as of 14:05, 22 October 2007 by Corinna (Talk | contribs)

Primers for specific ends of the dhfr1-fragment

pr-Kpn1-dhfr1: 5' TAATTAGGTA CCCAATTCCG GTTGTTC 3'

pf-Sph-h-dhfr1: 5' TATCGACGCA TGCACCATCA CCATCACCAT ATGGTTCGAC CATTGAAC 3'

Primers for calmodulin-construct with different linkerlengths

forward primers:

pf-calmo-KpnI-linker2: 5' TATCGACGGT ACCGGCGGTG AGCAGATTGC AGAGTTCAAA G 3'
pf-calmo-KpnI-linker3: 5' TATCGACGGT ACCGGCGGTG GCGAGCAGAT TGCAGAGTTC AAAG 3'
pf-calmo-KpnI-linker4: 5' TATCGACGGT ACCGGCGGTG GCGGTGAGCA GATTGCAGAG TTCAAAG 3'
pf-calmo-KpnI-linker6: 5' TATCGACGGT ACCGGCGGTG GCTCTGGTGG CGAGCAGATT GCAGAGTTCA AAG 3'
pf-calmo-KpnI-linker9: 5' TATCGACGGT ACCGGCGGTG GCGGTTCTGG CGGTGGCGGT GAGCAGATTG CAGAGTTCAA AG 3'

Retrieved from "http://2007.igem.org/wiki/index.php/Freiburg07/primers"