Week 16
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Revision as of 09:23, 23 October 2007 by Silvia.tamarri2 (Talk | contribs)
- 10/15/07
- O/N ligations for:
- -[http://partsregistry.org/Part:BBa_I763019 I763019];
- -[http://partsregistry.org/Part:BBa_I763039 I763039].
- 10/16/07
- Transformation of [http://partsregistry.org/Part:BBa_I763019 I763019] and I763039.
- We inoculate:
- -I763005;
- -I763015;
- -I763004.
- Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.
- 10/17/07
- Miniprep of:
- -I763005;
- -I763015;
- -I763004.
- We inoculate:
- -I763029;
- -I763039 (high copy).
- Control Digestion for results impagination (X/P):
- - I763020;
- - I763004;
- - I763011;
- - I763007 (on high copy plasmid);
- - I763007 (on low copy plasmid);
- - I763033;
- - I763015;
- - I763012;
- - I763005;
- - I763031.
- 10/18/07
- Miniprep:
- -[http://partsregistry.org/Part:BBa_I763029 I763029];
- -[http://partsregistry.org/Part:BBa_I763039 I763039](high copy).
- Digestion for:
- -[http://partsregistry.org/Part:BBa_I763029 I763029] (E/P)
- Band extraction of:
- -[http://partsregistry.org/Part:BBa_I763029 I763029] (E/P)
- Ligation for:
- -[http://partsregistry.org/Part:pSB4A3 pSB4A3] (E/P)
- -[http://partsregistry.org/Part:BBa_I763029 I763029] (E/P)
- Ligation for:
- -[http://partsregistry.org/Part:BBa_I763004 I763004] (X/P)
- -[http://partsregistry.org/Part:BBa_I763026 I763026] (S/P)
- Control Digestion for results impagination:
- - [http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
- - [http://partsregistry.org/Part:BBa_I763016 I763016] (high copy plasmid);
- - [http://partsregistry.org/Part:BBa_I763036 I763036];
- - [http://partsregistry.org/Part:BBa_I763019 I763019];
- - [http://partsregistry.org/Part:BBa_I763032 I763032];
- - [http://partsregistry.org/Part:BBa_I763025 I763025] (low copy plasmid);
- - [http://partsregistry.org/Part:BBa_I763025 I763025] (high copy plasmid);
- - [http://partsregistry.org/Part:BBa_I763026 I763026];
- - [http://partsregistry.org/Part:BBa_I763035 I763035].
- 10/19/07
- Transformation:
- -[http://partsregistry.org/Part:BBa_I763040 I763040] (on high copy plasmid);::
- -[http://partsregistry.org/Part:BBa_I763039 I763039];
- -[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (low copy plasmid).
