Tokyo/Works/Assay2
From 2007.igem.org
Works top 0.Hybrid promoter 1.Formulation 2.Assay1 3.Simulation 4.Assay2 5.Future works
Fomulationで建てた式に必要なパラメータをAssay１により実験的に求めた。それを導入した結果を解析したところ、以下の実験の必要性が浮かび上がった。
Parameters for the equations in Formulation <←link> have been experimentally determined in Assay1 <←link>. Analysing the result, the following experiments were turned out to be necessary.
Activation check by cell-produced AHL
Purpose
To check if worker E. coli (Sender) can produce enough AHL for our model to work by using different copy numbers of plasmids.
Result & Conclusion
Not only high copy number plasmid pSB1, also low copy number plasmid pSB4 and pBR produced enough AHL to activate the LacI hybrid promoter in other cells. Especially, pBR remarkably produced AHL in the present experiment.
