Virginia Tech/plasmid construct

The reporter plasmid the two different fluorescent genes are each on opposite sides of the right promoter of the λ switch.

Our original plan was to build our plasmid in two ways.

1) A bistable promoter derived from the λ switch (see Designing the Plasmid)

2) Two fluorescent protein genes from the registry

3) A vector backbone from the registry

While constructing our plasmid, we decided it would be interesting to sequence the registry in order to add more quality control. Other members of our lab undertook this project. More details are here.

Our more traditional plan involved synthesizing the plasmid. We knew this would be a quicker way to get the plasmid so that we could begin testing it and obtain experimental data for our model. The results from this approach did not turn out as we expected. We were delayed in finishing the design and having it synthesized.