Bologna University/M9 medium
From 2007.igem.org
Preparation instructions:
- 1. Dissolve 56.4g in 1L of distilled water.
- 2. Autoclave for 15min at 121°C.
This convenient 5x concentrate can be stored and diluted as needed to prepare 5L of 1x M9 minimal salts. For M9 minimal medium:
- 1. Aseptically dilute 200mL of M9 minimal salts, 5x [ ] with 800mL of sterile water, if necessary, cool to 45-50°C.
- 2. Aseptically add 20mL of sterile 1M glucose and 2mL of sterile 1M magnesium solfate to prepare 1L of M9 minimal medium.
- 3. If desired aseptically add 0.1mL of 1M sterile calcium chloride to the M9 minimal medium. M9 minimal medium may also be supplemented with the appropriate amino acids.
