From 2007.igem.org

Contents 1 AHL assay 1.1 Purpose1: 1.2 Samples: 1.3 Procedure: 1.4 Result & conclusion: 2

AHL assay

Purpose1:

To see how precisely we determine the fluorescence from GFP by removing LB media.

Samples:

placQI + GFP in pTrc99A (pos. con.)
A4 ΔP+ GFP (neg. con.)

Procedure:

AHL assay Standard protocol
Make 200 ul of mixtures of pos. con. and neg. con.
The mixture ratios of pos. con. and neg. con. are
(100:0), (70:30), (50:50), (30:70), (10:90), (3:97), (1:99)
Wash

Result & conclusion:

In proportion to the percentages of GFP expressing cells, fluorescence was increased as shown in Fig. 1.
This indicates that the "wash" process well removes the other factors influencing the fluorescence.

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