Tianjin/FLIP-FLOP

Design

"Flip-flop" is the common name given to two-state devices which offer basic memory for sequential logic operations. Flip-flops are widely used for digital data storage and transfer as well as in banks called "registers" for the storage of binary numerical data. Based on the conception of "Flip-flop" and synthetic biology, we designed the Genetically RS FLIP-FLOP whose output signal is regulated by additional input signal. Besides this, we modulate the performance of Genetically RS FLIP-FLOP to optimize our original design.

1.Introduction to the logic rules of our flip-flop


The logic principle of our design is shown above. Unless the input signal transfer from one stable condition to another ( such as 0 to 1 or 1 to 0), the output signal would change into 1, otherwise it would maintain 0. Thus, the immediate response to emergency (input change) enables our design to detect signal variation in a short time, which is beneficial for process control because of its time-saving character.

2.Construction of Biological Circuit

Modeling

1.Construction of Mathematical Model

2.Model Result
2.1Model of the whole system
2.1Change the degradation speed of AHL

Experiment

1.Ligation Strategy and Parts Reservoir