BerkiGEM2007-SamanthaConstructionFile9

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Revision as of 19:00, 20 June 2007 by Samanthaliang (Talk | contribs)

[rbs][Cre]
rbs library - want a weak rbs actually
3 Different Cre variants (ATG Bca1117, GTG I716212 and TTG I716213)


ATG Cre variants

Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716224.

Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small)
Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large)
Plate on KanAmp
Product is pBca9145-I716225.


GTG Cre variants

Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716226.

Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small)
Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)
Plate on KanAmp
Product is pBca9145-I716227.


TTG Cre variants

Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716228.

Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small)
Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large)
Plate on KanAmp
Product is pBca9145-I716229.