From 2007.igem.org

10/15/07

• O/N ligations for:

-[http://partsregistry.org/Part:BBa_I763019 I763019];

-[http://partsregistry.org/Part:BBa_I763039 I763039].

10/16/07

• Transformation of [http://partsregistry.org/Part:BBa_I763019 I763019] and [http://partsregistry.org/Part:BBa_I763039 I763039].
• We inoculate:

-[http://partsregistry.org/Part:BBa_I763005 I763005];

-[http://partsregistry.org/Part:BBa_I763015 I763015];

-[http://partsregistry.org/Part:BBa_I763004 I763004].

• Trasformation of [http://partsregistry.org/Part:BBa_I763019 I763019], subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.

10/17/07

• Miniprep of:

-[http://partsregistry.org/Part:BBa_I763005 I763005];

-[http://partsregistry.org/Part:BBa_I763015 I763015];

-[http://partsregistry.org/Part:BBa_I763004 I763004].

• We inoculate:

-[http://partsregistry.org/Part:BBa_I763029 I763029];

-[http://partsregistry.org/Part:BBa_I763039 I763039] (high copy).

• Control Digestion of the following Minipreps for results impagination:

-[http://partsregistry.org/Part:BBa_I763020 I763020];

-[http://partsregistry.org/Part:BBa_I763004 I763004];

-[http://partsregistry.org/Part:BBa_I763011 I763011];;

-[http://partsregistry.org/Part:BBa_I763007 I763007] (on high copy plasmid);

-[http://partsregistry.org/Part:BBa_I763007 I763007] (on low copy plasmid);

-[http://partsregistry.org/Part:BBa_I763033 I763033];

-[http://partsregistry.org/Part:BBa_I763015 I763015];

-[http://partsregistry.org/Part:BBa_I763012 I763012];

-[http://partsregistry.org/Part:BBa_I763005 I763005];

-[http://partsregistry.org/Part:BBa_I763031 I763031].

• Gel electrophoresis of the fragments above.

10/18/07

• Miniprep:

-[http://partsregistry.org/Part:BBa_I763029 I763029];

-[http://partsregistry.org/Part:BBa_I763039 I763039] (high copy).

• Digestion for:

-[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1)

• Band extraction of:

-[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1)

• Ligation for:

-[http://partsregistry.org/Part:pSB4A3 pSB4A3] (Eco/Pst1)

-[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1)

• Ligation for:

-[http://partsregistry.org/Part:BBa_I763004 I763004] (Xba/Pst1)

-[http://partsregistry.org/Part:BBa_I763026 I763026] (Spe/Pst1)

• Control Digestion of the following Minipreps for results impagination:

- [http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);

- [http://partsregistry.org/Part:BBa_I763016 I763016] (high copy plasmid);

- [http://partsregistry.org/Part:BBa_I763036 I763036];

- [http://partsregistry.org/Part:BBa_I763019 I763019];

- [http://partsregistry.org/Part:BBa_I763032 I763032];

- [http://partsregistry.org/Part:BBa_I763025 I763025] (low copy plasmid);

- [http://partsregistry.org/Part:BBa_I763025 I763025] (high copy plasmid);

- [http://partsregistry.org/Part:BBa_I763026 I763026];

- [http://partsregistry.org/Part:BBa_I763035 I763035].

• Gel electrophoresis of the fragments above.

10/19/07

• Transformation:

-[http://partsregistry.org/Part:BBa_I763034 I763034] (on high copy plasmid);

-[http://partsregistry.org/Part:BBa_I763039 I763039];

-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (low copy plasmid).

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