Melbourne/prefixsuffix primers
From 2007.igem.org
< Melbourne
Revision as of 16:14, 26 October 2007 by PhillipDodson (Talk | contribs)
- Melting temperatures of the overlaps to the gene sequence range from 82degC to 90 degC
- GC content ranges from 42% to 61%
- The primers not only allow all ORFs to be extracted but can also be used to sequence the whole polycystronic gene.
Name | Forward primer Sequence: 5’-…..-3’ |
---|---|
NL26-F | GAATTCGCGGCCGCTTCTAGATAGAAGGATTTACGCGC |
GvpA-F | GAATTCGCGGCCGCTTCTAGATGAGTATTCAAAAAAGTACAG |
GvpP-F | GAATTCGCGGCCGCTTCTAGATGTCAACAACTGATAACAATATG |
NL29-F | GAATTCGCGGCCGCTTCTAGGAAAGAAGCTGGAGAAAAC |
GvpQ-F | GAATTCGCGGCCGCTTCTAGATGGATAAAAAAGATGTGGAG |
GvpB-F | GAATTCGCGGCCGCTTCTAGATGTCTATTCAAAAAAGTACTAATAG |
GvpR-F | GAATTCGCGGCCGCTTCTAGATGGAAATTAAAAAAATTATGCAAGC |
GvpN-F | GAATTCGCGGCCGCTTCTAGATGACCGTCTTAACAGAC |
GvpF-F | GAATTCGCGGCCGCTTCTAGATGAGTGAAACAAACGAAACAGG |
GvpG-F | GAATTCGCGGCCGCTTCTAGATGCTTCACAAATTAGTAACCGC |
GvpL-F | GAATTCGCGGCCGCTTCTAGATGGGAGAATTACTGTATTTATAC |
GvpS-F | GAATTCGCGGCCGCTTCTAGATGTCTCTTAAACAATCCATGG |
GvpK-F | GAATTCGCGGCCGCTTCTAGATGCAACCGGTCAGCC |
GvpJ-F | GAATTCGCGGCCGCTTCTAGATGGCAGTCGAACATAATATG |
GvpT-F | GAATTCGCGGCCGCTTCTAGATGGCAACTGAAACAAAATTAG |
GvpU-F | GAATTCGCGGCCGCTTCTAGATGAGTACAGGCCCTTC |
Name | Reverse primer Sequence: 5’-…..-3’ |
---|---|
NL26-R | AAGGCTGCAGCGGCCGCTACTAGTACTGCAGCAAGCAGAAGC |
GvpA-R | AAGGCTGCAGCGGCCGCTACTAGTATTAAAAGCTAAGCCCTGC |
GvpP-R | AAGGCTGCAGCGGCCGCTACTAGTATTACTTCTTCGCGTCCATG |
GvpQ-R | AAGGCTGCAGCGGCCGCTACTAGTATTATGATCCCATTGTTTTTAAATC |
GvpB-R | AAGGCTGCAGCGGCCGCTACTAGTATTAAATACTAAAACGCGGCTG |
GvpR-R | AAGGCTGCAGCGGCCGCTACTAGTATTATGCTTCAATGCCAATGG |
GvpN-R | AAGGCTGCAGCGGCCGCTACTAGTATTACTTTTCTTCAGTGTCTATATTC |
GvpF-R | AAGGCTGCAGCGGCCGCTACTAGTATTATTTCTCTTCTACTTTTAGGC |
GvpG-R | AAGGCTGCAGCGGCCGCTACTAGTACTAGGATTCCTCATTTCTTTTTTGTG |
GvpL-R | AAGGCTGCAGCGGCCGCTACTAGTATTACGCAAAGCTCGAGAAATG |
GvpS-R | AAGGCTGCAGCGGCCGCTACTAGTATCAGGATCCAAGTGGATTC |
GvpK-R | AAGGCTGCAGCGGCCGCTACTAGTATTAAAGCAGGCTGCCTAGC |
GvpJ-R | AAGGCTGCAGCGGCCGCTACTAGTATTAACGTTTCGTTTCTATTTTTTCTTC |
GvpT-R | AAGGCTGCAGCGGCCGCTACTAGTATTAGGCTGTTGTATCGTCTTC |
GvpU-R | AAGGCTGCAGCGGCCGCTACTAGTATTATGAACTCTTTTTACTCGTTG |