Chiba/Communication

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Chiba logo.png

Introduction | Project Design ( 1.Sticky Hands | 2.Communication | 3.Size Control ) | Making Marimos | Our Goal || Team Members | メンバ連絡簿


Communication

Our Aim

Fig1. Micro.

Our aim is that bacterias stick around the bacterias core. We make bacteria sending a signal(sender) and bacteria receiving a signal(receiver). Senders sticking each other in advance send a signal to receivers, which grow stickey hands. In this way, bacterias stick. We use Quorum sensing as a signal.
違う遺伝子回路を持つ2つの細胞(sender&receiver)に分ける.あらかじめお互い結合しているSenderが,周りのreceiverにシグナルを与えてstickey handsを生やす.そして合体.このシグナルにはQuorum sensingを利用する.

About Quorum Sensing

Fig2.

Quorum Sinsing is cell-to-cell signaling action of bacteria. Detect the cell density of same species and coordinating the behavior of cells within a population. Species of Gram-Negativfe signaling information transfer molecule (autoinducer) is acyl homoserine lactone (AHL).When AHL concentration exceed threshold thus the cell population over a certain number, AHL regulated promoter is activated transcription.

Detail geneticaly circuit discribed in Fig2.

クオラムセンシングとは,バクテリアが細胞間で行うシグナル伝達のシステムである.自分と同種の菌の生息密度を感知して、それに応じて物質の産生をコントロールする.情報伝達に使用している化合物(オートインデューサー)はグラム陰性菌の場合AHLである.AHLの濃度がある値以上になる,すなわち細胞密度がある値を超えるとAHL依存性プロモータ(LuxP)のスイッチがオンになり,遺伝子が発現される. 詳しい遺伝子回路は図に示す通り.

  • more about quorum sensing

[http://partsregistry.org/Featured_Parts:Cell-Cell-Signaling Cell-Cell-Signaling]

[http://www.che.caltech.edu/groups/fha/quorum.html About Quorum sensing]

Experiments

Making Recievers

We Made

  • ver.1(BBa_T729006)
    • BBa I729006 circuit.jpg
  • ver2.(BBa_I729005)
    • BBa I729005 circuit.jpg

From Biobrick

  • ver.0(BBa_T9002)
    • T9002 circuit.jpg

Future Work

  • ver3
    • Rec inv aiia.jpg

Making Senders

We Made

  • MetK Sender

MetK circuit.jpg

From Biobrick

  • Sender(BBa_S03623)

BBa s03623.jpg

Future Work

  • Light Sensing Sender (Light -> AHL)

Light sensing sender.jpg

futureでLuxIもスイッチできた方が魅力的だと思うんだ。∵ReceiverにSenderを混ぜるのではなく,初めからReceiverとSenderを混ぜた状態で凝集させることができる。ex.)Photoswitch etc... by tashiro