June 21
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Contents |
Experiments
Microscopy:
- Open loop trial - 50 ng/ml aTc
- Full range induction of pL CFP for equivalence. NOT final because OD was >0.1 (~0.4-1.0)
Analysis
Microscopy:
- Open loop trial - 50 ng/ml aTc
- For both cases of AI (grown at 50 ng/mL and ODs 0.2/2), make Scatter plots of CFP vs LuxR.YFP for all 6 concentrations of IPTG.
- A combined plot of Mean and S.D of CFP and LuxR.Y having points from OD 0.2 in one colour and OD 2 in the other colour.
Scatter Plots Green : AI OD 2 Blue : AI OD 0.2
| File:Scatter openloop J21.jpg
Mean and Standard Error Plots Red : AI OD 2 Blue : AI OD 0.2 for CFP , YFP, RFP Linear and Log space InferenceaTc being an antibiotic may have adverse effects on the cell at high concentrations such as 100 ng/ml. Growth rate is reduced and cells are killed. These factors may in turn affect the expression of the fluorescence gene which may lead to a drop in mean fluorescence intensity at high concentration levels. Discussions |
