OtsA-construction file

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PCR otsAF/otsAIR on otsA (413 bp, gp=A) PCR otsAIF/otsAR on otsA (1077 bp, gp=B)

PCR otsAF/otsAR on A+B (1456 bp BglII/XhoI) Digest pBca9145-Bca1144 (BglII/XhoI 2054+910, L) Product pBca9145-BBa I716301

otsAF forward BglII site anneals to otsA cgtaaAGATCTATGAGTCGTTTAGTCGTAGTATC otsAIF Removing BamHI site in otsA CGATGACATTATCTGGATtCACGATTATCACCTG otsAIR Removing BamHI site in otsA CAGGTGATAATCGTGaATCCAGATAATGTCATCG otsaR reverse XhoI site anneals to otsA cgtatCTCGAGttaGGATCCTTACGCAAGCTTTGGAAAGGTAGC

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