BerkiGEM2007-AustinConstructionFile24

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Revision as of 21:49, 2 July 2007 by AustinDay (Talk | contribs)

Alpha:
PCR CA1150/CA1099R on I716077 (1727 Bp)
Digest (Bsa1/Bgl2) and ligate with beta portion.

Beta:

PCR CA1099R/D58 on Part Ptrc-I716081
Digest (Bsa1/BamH1) and ligate with alpha portion.


Product is pTRC-I716074 (best yield)

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