Wisconsin/Protocol:Agarose Gel

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1.2% Agarose Gel preparation

  • 8 wells
  • 600mg Agarose
  • 50mL TBE
  • 1uL 10mg/ml EtBr

Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box.

  • 2uL DNA
  • 12uL ddH2O
  • 4uL 5x Loading Dye
  • 4uL DNA Ladder for reference

Add water if DNA is less than 10uL and make sure #uL of 1x loading dye = #uL total. Mix content in tube before adding it to well. Also make sure gel box is submerged in TAE/TBE before adding DNA.

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