BerkiGEM2007-AustinConstructionFile24
From 2007.igem.org
Alpha:
PCR CA1150/CA1099R on 9145-I716077 (1727 Bp)
Digest (Bsa1/Bgl2) and ligate with beta portion.
Beta:
PCR CA1099F/G00101 on Part 9145-I716081
Digest (Bsa1/BamH1) and ligate with alpha portion.
Product is 9145-I716074 (best yield)